C2, An Unusual Filamentous Bacterial Virus: Protein Sequence and Conformation, DNA Size and Conformation, and Nucleotide/Subunit Ratio
Ημερομηνία
1995Source
BiochemistryVolume
34Pages
4077-4087Google Scholar check
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Εμφάνιση πλήρους εγγραφήςΕπιτομή
Inovirus C2 is 1295 nm long and 6.8 nm in diameter, and its mass is 24 million Da. Its genome is a topologically circular, single-stranded DNA molecule of 8100 nucleotides. The DNA is packed in the virion as two antiparallel strands, with a rise per nucleotide in each strand of 3.2 Å it can be assigned spectroscopic properties like those of base-stacked, right-handed, double-stranded DNA. The stoichiometric ratio (n/s) of nucleotides to subunits of the major coat protein is close to 2. The protein subunit contains 52 amino acids, and the DNA sequence of its gene does not encode a signal peptide. The protein conformation in the virion is helical, mostly α-helix with perhaps some 310-helix. The amino acid sequence of the DNA interaction domain of the subunit is unique among Inovirus species. On the basis of its coat protein sequence and available theories of helical symmetry in such structures, C2 appears to be either an unusual member of filamentous virus symmetry class II or the defining member of a new symmetry class. © 1995, American Chemical Society. All rights reserved.
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