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Export of infectious particles by Escherichia coli transfected with the RF DNA of Pf1, a virus of Pseudomonas aeruginosa strain K

dc.contributor.authorKostrikis, Leontios G.en
dc.contributor.authorReisberg, S. A.en
dc.contributor.authorSimon, M. N.en
dc.contributor.authorWall, J. S.en
dc.contributor.authorDay, L. A.en
dc.creatorKostrikis, Leontios G.en
dc.creatorReisberg, S. A.en
dc.creatorSimon, M. N.en
dc.creatorWall, J. S.en
dc.creatorDay, L. A.en
dc.date.accessioned2019-11-04T12:52:14Z
dc.date.available2019-11-04T12:52:14Z
dc.date.issued1991
dc.identifier.issn0950-382X
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/53203
dc.description.abstractPf1 is a filamentous, single‐stranded DNA virus that has Pseudomas aeruginosa (strain K) as host. It is the longest of the filamentous bacterial viruses, and the DNA within it has the most extended conformation known. Pfl virus cannot infect Escherichia coli (strain MM294) cells, but when these cells are transfected with the double‐stranded replicative form of Pt1 DNA (RF DNA, 7.35 kb), they export low levels of infectious particles that create plaques on lawns of P. aeruginosa. Several different structural species, at least two of which are infectious, are exported. One of them, called Epf1, has virtually the same structure as Pf1, but the amount of Epf1 exported by E. coli is 104 lower than the amount of Pf1 exported by P. aeruginosa. The results imply that host factors affect not only the efficiency of virus assembly and export, but also the actual structures of the species exported. Copyright © 1991, Wiley Blackwell. All rights reserveden
dc.sourceMolecular microbiologyen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0025983830&doi=10.1111%2fj.1365-2958.1991.tb01973.x&partnerID=40&md5=ffb3b9c4b8cb51eebe196657259d814c
dc.subjectarticleen
dc.subjectpriority journalen
dc.subjectnonhumanen
dc.subjectDNA, Viralen
dc.subjectSupport, U.S. Gov't, P.H.S.en
dc.subjectTransfectionen
dc.subjectescherichia colien
dc.subjectSupport, U.S. Gov't, Non-P.H.S.en
dc.subjectbacteriophageen
dc.subjectMicroscopy, Electronen
dc.subjectBacteriophagesen
dc.subjectpseudomonas aeruginosaen
dc.subjectvirusen
dc.subjectCapsiden
dc.subjectdna transferen
dc.subjectVirionen
dc.titleExport of infectious particles by Escherichia coli transfected with the RF DNA of Pf1, a virus of Pseudomonas aeruginosa strain Ken
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1111/j.1365-2958.1991.tb01973.x
dc.description.volume5
dc.description.startingpage2641
dc.description.endingpage2647
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeArticleen
dc.description.notes<p>Cited By :6</p>en
dc.source.abbreviationMol.Microbiol.en
dc.contributor.orcidKostrikis, Leontios G. [0000-0002-5340-7109]
dc.gnosis.orcid0000-0002-5340-7109


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