dc.contributor.author | Kostrikis, Leontios G. | en |
dc.contributor.author | Touloumi, G. | en |
dc.contributor.author | Karanicolas, R. | en |
dc.contributor.author | Pantazis, N. | en |
dc.contributor.author | Anastassopoulou, C. G. | en |
dc.contributor.author | Karafoulidou, A. | en |
dc.contributor.author | Goedert, J. J. | en |
dc.contributor.author | Hatzakis, Angelos E. | en |
dc.creator | Kostrikis, Leontios G. | en |
dc.creator | Touloumi, G. | en |
dc.creator | Karanicolas, R. | en |
dc.creator | Pantazis, N. | en |
dc.creator | Anastassopoulou, C. G. | en |
dc.creator | Karafoulidou, A. | en |
dc.creator | Goedert, J. J. | en |
dc.creator | Hatzakis, Angelos E. | en |
dc.date.accessioned | 2019-11-04T12:52:14Z | |
dc.date.available | 2019-11-04T12:52:14Z | |
dc.date.issued | 2002 | |
dc.identifier.issn | 0022-538X | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53205 | |
dc.description.abstract | There are several forms of human immunodeficiency virus type 1 (HIV-1) DNA in peripheral blood T cells and lymph nodes in untreated HIV-1-infected individuals and in patients whose plasma HIV-1 RNA levels are suppressed by long-term combination antiretroviral therapy. However, it remains to be established whether the concentration of HIV-1 DNA in cells predicts the clinical outcome of HIV-1 infection. In this report, we measured the concentration of HIV-1 DNA forms which has undergone the second template switch (STS DNA) and 2-long-terminal-repeat DNA circles in peripheral blood mononuclear cell (PBMC) samples. To do this, we used molecular-beacon-based real-time PCR assays and studied 130 patients with hemophilia in the Multi-center Hemophilia Cohort Study. We assessed the influence of baseline HIV-1 STS DNA levels on the progression of HIV-1 disease in the absence of combination antiretroviral therapy by Kaplan-Meier and Cox regression analysis. Among the patients who progressed to AIDS, the median levels (interquartile ranges) of STS HIV-1 DNA in PBMC were significantly higher than those of patients who remained AIDS free during the 16 years of follow-up (1,017 [235 to 6,059] and 286 [31 to 732] copies per 106 PBMC, respectively | en |
dc.description.abstract | P < 0.0001). Rates of progression to death and development of AIDS varied significantly (log rank P < 0.001) by quartile distribution of HIV-1 STS DNA levels. After adjustment for age at seroconversion, baseline CD4+ T-cell counts, plasma viral load, and T-cell-receptor excision circles, the relative hazards (RH) of death and AIDS were significantly increased with higher HIV-1 STS DNA levels (adjusted RH, 1.84 [95% confidence interval {CI}, 1.30 to 2.59] and 2.62 [95% CI, 1.75 to 3.93] per 10-fold increase per 106 PBMC, respectively). HIV-1 STS DNA levels in each individual remained steady in longitudinal PBMC samples during 16 years of follow-up. Our findings show that the concentration of HIV-1 STS DNA in PBMC complements the HIV-1 RNA load in plasma in predicting the clinical outcome of HIV-1 disease. This parameter may have important implications for understanding the virological response to combination antiretroviral therapy. | en |
dc.source | Journal of virology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036784560&doi=10.1128%2fJVI.76.20.10099-10108.2002&partnerID=40&md5=cc538dc9f61700873294682ab28b3861 | |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | major clinical study | en |
dc.subject | Disease Progression | en |
dc.subject | Predictive Value of Tests | en |
dc.subject | priority journal | en |
dc.subject | male | en |
dc.subject | HIV Infections | en |
dc.subject | death | en |
dc.subject | Kaplan Meier method | en |
dc.subject | highly active antiretroviral therapy | en |
dc.subject | lymphocyte count | en |
dc.subject | virus load | en |
dc.subject | virus RNA | en |
dc.subject | polymerase chain reaction | en |
dc.subject | Viral Load | en |
dc.subject | mononuclear cell | en |
dc.subject | Human immunodeficiency virus 1 | en |
dc.subject | HIV-1 | en |
dc.subject | acquired immune deficiency syndrome | en |
dc.subject | seroconversion | en |
dc.subject | hemophilia | en |
dc.subject | virus DNA | en |
dc.subject | DNA, Viral | en |
dc.subject | RNA, Viral | en |
dc.subject | long terminal repeat | en |
dc.subject | DNA determination | en |
dc.subject | Leukocytes, Mononuclear | en |
dc.subject | T lymphocyte receptor | en |
dc.subject | Hemophilia A | en |
dc.subject | Templates, Genetic | en |
dc.title | Quantitation of human immunodeficiency virus type 1 DNA forms with the second template switch in peripheral blood cells predicts disease progression independently of plasma RNA load | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1128/JVI.76.20.10099-10108.2002 | |
dc.description.volume | 76 | |
dc.description.startingpage | 10099 | |
dc.description.endingpage | 10108 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :73</p> | en |
dc.source.abbreviation | J.Virol. | en |
dc.contributor.orcid | Kostrikis, Leontios G. [0000-0002-5340-7109] | |
dc.gnosis.orcid | 0000-0002-5340-7109 | |