dc.contributor.author | Santos-Rosa, H. | en |
dc.contributor.author | Kirmizis, Antonis | en |
dc.contributor.author | Nelson, C. | en |
dc.contributor.author | Bartke, T. | en |
dc.contributor.author | Saksouk, N. | en |
dc.contributor.author | Cote, J. | en |
dc.contributor.author | Kouzarides, T. | en |
dc.creator | Santos-Rosa, H. | en |
dc.creator | Kirmizis, Antonis | en |
dc.creator | Nelson, C. | en |
dc.creator | Bartke, T. | en |
dc.creator | Saksouk, N. | en |
dc.creator | Cote, J. | en |
dc.creator | Kouzarides, T. | en |
dc.date.accessioned | 2019-11-04T12:52:37Z | |
dc.date.available | 2019-11-04T12:52:37Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 1545-9993 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53366 | |
dc.description.abstract | Induction of gene expression in yeast and human cells involves changes in the histone modifications associated with promoters. Here we identify a histone H3 endopeptidase activity in Saccharomyces cerevisiae that may regulate these events. The endopeptidase cleaves H3 after Ala21, generating a histone that lacks the first 21 residues and shows a preference for H3 tails carrying repressive modifications. In vivo, the H3 N terminus is clipped, specifically within the promoters of genes following the induction of transcription. H3 clipping precedes the process of histone eviction seen when genes become fully active. A truncated H3 product is not generated in yeast carrying a mutation of the endopeptidase recognition site (H3 Q19A L20A) and gene induction is defective in these cells. These findings identify clipping of H3 tails as a previously uncharacterized modification of promoter-bound nucleosomes, which may result in the localized clearing of repressive signals during the induction of gene expression. © 2009 Nature America, Inc. All rights reserved. | en |
dc.source | Nature Structural and Molecular Biology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-58149260198&doi=10.1038%2fnsmb.1534&partnerID=40&md5=ca1de2f1a942ebac909f933da1186814 | |
dc.subject | article | en |
dc.subject | controlled study | en |
dc.subject | priority journal | en |
dc.subject | promoter region | en |
dc.subject | nonhuman | en |
dc.subject | gene expression | en |
dc.subject | genetic transcription | en |
dc.subject | enzyme activity | en |
dc.subject | in vivo study | en |
dc.subject | Transcription, Genetic | en |
dc.subject | amino terminal sequence | en |
dc.subject | gene induction | en |
dc.subject | Saccharomyces cerevisiae | en |
dc.subject | RNA, Messenger | en |
dc.subject | protein cleavage | en |
dc.subject | serine proteinase | en |
dc.subject | Endopeptidases | en |
dc.subject | proteinase | en |
dc.subject | histone H3 | en |
dc.subject | Histones | en |
dc.subject | Gene Expression Regulation, Fungal | en |
dc.subject | Saccharomyces cerevisiae Proteins | en |
dc.subject | nucleosome | en |
dc.subject | alanine | en |
dc.subject | Genes, Fungal | en |
dc.subject | Promoter Regions, Genetic | en |
dc.subject | RNA, Fungal | en |
dc.title | Histone H3 tail clipping regulates gene expression | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1038/nsmb.1534 | |
dc.description.volume | 16 | |
dc.description.startingpage | 17 | |
dc.description.endingpage | 22 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :70</p> | en |
dc.source.abbreviation | Nat.Struct.Mol.Biol. | en |
dc.contributor.orcid | Kirmizis, Antonis [0000-0002-3748-8711] | |
dc.gnosis.orcid | 0000-0002-3748-8711 | |