Stable transfection of an estrogen receptor beta cDNA isoform into MDA-MB-231 breast cancer cells
dc.contributor.author | Tonetti, D. A. | en |
dc.contributor.author | Rubenstein, R. | en |
dc.contributor.author | DeLeon, M. | en |
dc.contributor.author | Zhao, H. | en |
dc.contributor.author | Pappas, S. G. | en |
dc.contributor.author | Bentrem, D. J. | en |
dc.contributor.author | Chen, B. | en |
dc.contributor.author | Constantinou, Andreas I. | en |
dc.contributor.author | Jordan, V. C. | en |
dc.creator | Tonetti, D. A. | en |
dc.creator | Rubenstein, R. | en |
dc.creator | DeLeon, M. | en |
dc.creator | Zhao, H. | en |
dc.creator | Pappas, S. G. | en |
dc.creator | Bentrem, D. J. | en |
dc.creator | Chen, B. | en |
dc.creator | Constantinou, Andreas I. | en |
dc.creator | Jordan, V. C. | en |
dc.date.accessioned | 2019-11-04T12:52:46Z | |
dc.date.available | 2019-11-04T12:52:46Z | |
dc.date.issued | 2003 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53416 | |
dc.description.abstract | We previously reported stable transfection of estrogen receptor alpha (ERα) into the ER-negative MDA-MB-231 cells (S30) as a tool to examine the mechanism of action of estrogen and antiestrogens [J. Natl. Cancer Inst. 84 (1992) 580]. To examine the mechanism of ERβ action directly, we have similarly created ERβ stable transfectants in MDA-MB-231 cells. MDA-MB-231 cells were stably transfected with ERβ cDNA and clones were screened by estrogen response element (ERE)-luciferase assay and ERβ mRNA expression was quantified by real-time RT-PCR. Three stable MDA-MB-231/ERβ clones were compared with S30 cells with respect to their growth properties, ability to activate ERE- and activating protein-1 (AP-1) luciferase reporter constructs, and the ability to activate the endogenous ER-regulated transforming growth factor alpha (TGFα) gene. ERβ6 and ERβ27 clones express 300-400-fold and the ERβ41 clone express 1600-fold higher ERβ mRNA levels compared with untransfected MDA-MB-231 cells. Unlike S30 cells, 17β-estradiol (E2) does not inhibit ERβ41 cell growth. ERE-luciferase activity is induced six-fold by E2 whereas neither 4-hydroxytamoxifen (4-OHT) nor ICI 182, 780 activated an AP-1-luciferase reporter. TGFα mRNA is induced in response to E2, but not in response to 4-OHT. MDA-MB-231/ERβ clones exhibit distinct characteristics from S30 cells including growth properties and the ability to induce TGFα gene expression. Furthermore, ERβ, at least in the context of the MDA-MB-231 cellular milieu, does not enhance AP-1 activity in the presence of antiestrogens. In summary, the availability of both ERα and ERβ stable breast cancer cell lines now allows us to compare and contrast the long-term consequences of individual signal transduction pathways. © 2003 Elsevier Ltd. All rights reserved. | en |
dc.source | Journal of Steroid Biochemistry and Molecular Biology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0242578587&doi=10.1016%2fj.jsbmb.2003.07.003&partnerID=40&md5=57df76a18149406c55951660f6d6c5c8 | |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | Breast Neoplasms | en |
dc.subject | controlled study | en |
dc.subject | cancer growth | en |
dc.subject | protein expression | en |
dc.subject | Cell Division | en |
dc.subject | Breast cancer | en |
dc.subject | cancer cell | en |
dc.subject | human cell | en |
dc.subject | messenger RNA | en |
dc.subject | reverse transcription polymerase chain reaction | en |
dc.subject | Tamoxifen | en |
dc.subject | fulvestrant | en |
dc.subject | Estrogen Receptor alpha | en |
dc.subject | concentration response | en |
dc.subject | protein folding | en |
dc.subject | Transcription, Genetic | en |
dc.subject | Receptors, Estrogen | en |
dc.subject | Transfection | en |
dc.subject | estradiol | en |
dc.subject | RNA, Messenger | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | molecular cloning | en |
dc.subject | complementary DNA | en |
dc.subject | DNA, Complementary | en |
dc.subject | Genes, Reporter | en |
dc.subject | reporter gene | en |
dc.subject | luciferase | en |
dc.subject | Gene Expression Regulation, Neoplastic | en |
dc.subject | cell activation | en |
dc.subject | 4 hydroxytamoxifen | en |
dc.subject | AP-1 | en |
dc.subject | DNA transfection | en |
dc.subject | Estrogen receptor beta | en |
dc.subject | estrogen responsive element | en |
dc.subject | MDA-MB-231 | en |
dc.subject | Response Elements | en |
dc.subject | Stable clones | en |
dc.subject | TGF alpha | en |
dc.subject | transcription factor AP 1 | en |
dc.subject | Transcription Factor AP-1 | en |
dc.subject | Transforming Growth Factor alpha | en |
dc.subject | transforming growth factor alpha receptor | en |
dc.title | Stable transfection of an estrogen receptor beta cDNA isoform into MDA-MB-231 breast cancer cells | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1016/j.jsbmb.2003.07.003 | |
dc.description.volume | 87 | |
dc.description.startingpage | 47 | |
dc.description.endingpage | 55 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :38</p> | en |
dc.source.abbreviation | J.Steroid Biochem.Mol.Biol. | en |
dc.contributor.orcid | Constantinou, Andreas I. [0000-0003-0365-1821] | |
dc.gnosis.orcid | 0000-0003-0365-1821 |
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