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In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography

dc.contributor.authorVinegoni, C.en
dc.contributor.authorPitsouli, Chrysoulaen
dc.contributor.authorRazansky, D.en
dc.contributor.authorPerrimon, N.en
dc.contributor.authorNtziachristos, V.en
dc.creatorVinegoni, C.en
dc.creatorPitsouli, Chrysoulaen
dc.creatorRazansky, D.en
dc.creatorPerrimon, N.en
dc.creatorNtziachristos, V.en
dc.date.accessioned2019-11-04T12:52:50Z
dc.date.available2019-11-04T12:52:50Z
dc.date.issued2008
dc.identifier.issn1548-7091
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/53433
dc.description.abstractWe report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.en
dc.sourceNature Methodsen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-37749036012&doi=10.1038%2fnmeth1149&partnerID=40&md5=83f454e9c1915a16dfc4cb19e0af85a2
dc.subjectarticleen
dc.subjectcontrolled studyen
dc.subjectpriority journalen
dc.subjectintermethod comparisonen
dc.subjecttissue sectionen
dc.subjectnonhumanen
dc.subjectAnimalsen
dc.subjectanimal tissueen
dc.subjectmolecular imagingen
dc.subjectImage Enhancementen
dc.subjectLightingen
dc.subjectstructure analysisen
dc.subjectin vivo studyen
dc.subjectDrosophila melanogasteren
dc.subjectmorphogenesisen
dc.subjectMicroscopy, Fluorescenceen
dc.subjectfluorescence microscopyen
dc.subjectdevelopmental stageen
dc.subjectanimal identificationen
dc.subjectfluorescence tomographyen
dc.subjectilluminationen
dc.subjectImaging, Three-Dimensionalen
dc.subjectPupaen
dc.subjectpupa (life cycle stage)en
dc.subjecttomographyen
dc.subjectTomography, Opticalen
dc.subjectwhole body imagingen
dc.titleIn vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomographyen
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1038/nmeth1149
dc.description.volume5
dc.description.startingpage45
dc.description.endingpage47
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeArticleen
dc.description.notes<p>Cited By :79</p>en
dc.source.abbreviationNat.Methodsen
dc.contributor.orcidPitsouli, Chrysoula [0000-0003-4074-9684]
dc.gnosis.orcid0000-0003-4074-9684


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