A monoclonal antibody against RAGE alters gene expression and is protective in experimental models of sepsis and pneumococcal pneumonia
dc.contributor.author | Christaki, Eirini | en |
dc.contributor.author | Opal, Steven M. | en |
dc.contributor.author | Jr, James C. Keith | en |
dc.contributor.author | Kessimian, Nubar | en |
dc.contributor.author | Palardy, J. E. | en |
dc.contributor.author | Parejo, N. A. | en |
dc.contributor.author | Tan, X. Y. | en |
dc.contributor.author | Piche-Nicholas, N. | en |
dc.contributor.author | Tchistiakova, L. | en |
dc.contributor.author | Vlasuk, G. P. | en |
dc.contributor.author | Shields, K. M. | en |
dc.contributor.author | Feldman, Jeffrey L. | en |
dc.contributor.author | LaVallie, Edward R. | en |
dc.contributor.author | Arai, Maya | en |
dc.contributor.author | Mounts, W. | en |
dc.contributor.author | Pittman, D. D. | en |
dc.creator | Christaki, Eirini | en |
dc.creator | Opal, Steven M. | en |
dc.creator | Jr, James C. Keith | en |
dc.creator | Kessimian, Nubar | en |
dc.creator | Palardy, J. E. | en |
dc.creator | Parejo, N. A. | en |
dc.creator | Tan, X. Y. | en |
dc.creator | Piche-Nicholas, N. | en |
dc.creator | Tchistiakova, L. | en |
dc.creator | Vlasuk, G. P. | en |
dc.creator | Shields, K. M. | en |
dc.creator | Feldman, Jeffrey L. | en |
dc.creator | LaVallie, Edward R. | en |
dc.creator | Arai, Maya | en |
dc.creator | Mounts, W. | en |
dc.creator | Pittman, D. D. | en |
dc.date.accessioned | 2018-06-22T09:52:50Z | |
dc.date.available | 2018-06-22T09:52:50Z | |
dc.date.issued | 2011 | |
dc.identifier.uri | https://gnosis.library.ucy.ac.cy/handle/7/41534 | |
dc.description.abstract | The RAGE (receptor for advanced glycation end products) is believed to play a role in sepsis by perpetuating inflammation. The interaction of RAGE with a variety of host-derived ligands that accumulate during stress and inflammation further induces the expression of RAGE. It was previously shown that a rat anti-RAGE monoclonal antibody protected mice from lethality in a cecal ligation and puncture model. We studied the effects of a humanized anti-RAGE monoclonal antibody in the murine pneumococcal pneumonia model of sepsis. Moreover, a gene expression analysis was performed in lung tissue of animals that underwent cecal ligation and puncture and treated with the rat anti-RAGE monoclonal antibody, compared with controls. Administration of humanized anti-RAGE mAb 6 h after intratracheal infection with Streptococcus pneumoniae improved mortality in BALB/c mice whether a 7.5 mg/kg (P < 0.01) or a 15 mg/kg dose (P < 0.01) was administered in combination with antibiotics. Gene expression analysis showed that many of the genes modulated by treatment with the anti-RAGE antibody were those that play an important role in regulating inflammation. Anti-RAGE monoclonal antibody offered a survival advantage to septic mice. This protective role in treated animals is supported by the observed gene expression profile changes of genes involved in sepsis and inflammation. Copyright © 2011 by the Shock Society. | en |
dc.language.iso | eng | en |
dc.source | Shock | en |
dc.subject | Article | en |
dc.subject | Controlled study | en |
dc.subject | Female | en |
dc.subject | Male | en |
dc.subject | Mortality | en |
dc.subject | Antibodies | en |
dc.subject | Monoclonal antibody | en |
dc.subject | Receptors | en |
dc.subject | Unclassified drug | en |
dc.subject | Nonhuman | en |
dc.subject | Gene expression | en |
dc.subject | Septic shock | en |
dc.subject | Transcriptomics | en |
dc.subject | Pneumonia | en |
dc.subject | Advanced glycation end product receptor | en |
dc.subject | Animals | en |
dc.subject | Mice | en |
dc.subject | Animal | en |
dc.subject | Animal experiment | en |
dc.subject | Animal model | en |
dc.subject | Disease models | en |
dc.subject | Kaplan-meier estimate | en |
dc.subject | Mouse | en |
dc.subject | Pneumococcal | en |
dc.subject | Streptococcus pneumonia | en |
dc.subject | Streptococcus pneumoniae | en |
dc.subject | Sepsis | en |
dc.subject | Immunologic | en |
dc.subject | Genetic transcription | en |
dc.subject | Inbred balb c | en |
dc.subject | Lung parenchyma | en |
dc.subject | Monoclonal | en |
dc.subject | Monoclonal antibody rage | en |
dc.subject | Moxifloxacin | en |
dc.subject | Pneumococcal pneumonia | en |
dc.subject | Receptor for advanced glycation end products (rage) | en |
dc.title | A monoclonal antibody against RAGE alters gene expression and is protective in experimental models of sepsis and pneumococcal pneumonia | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1097/SHK.0b013e31820b2e1c | |
dc.description.volume | 35 | |
dc.description.issue | 5 | |
dc.description.startingpage | 492 | |
dc.description.endingpage | 498 | |
dc.author.faculty | Ιατρική Σχολή / Medical School | |
dc.author.department | Ιατρική Σχολή / Medical School | |
dc.type.uhtype | Article | en |
dc.contributor.orcid | Christaki, Eirini [0000-0002-8152-6367] | |
dc.gnosis.orcid | 0000-0002-8152-6367 |
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