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dc.contributor.authorDova, L.en
dc.contributor.authorPentheroudakis, Georgeen
dc.contributor.authorGolfinopoulos, Vassilisen
dc.contributor.authorMalamou-Mitsi, Vassiliki D.en
dc.contributor.authorGeorgiou, I.en
dc.contributor.authorVartholomatos, G.en
dc.contributor.authorNtemou, A.en
dc.contributor.authorFountzilas, Georgeen
dc.contributor.authorPavlidis, Nicholasen
dc.creatorDova, L.en
dc.creatorPentheroudakis, Georgeen
dc.creatorGolfinopoulos, Vassilisen
dc.creatorMalamou-Mitsi, Vassiliki D.en
dc.creatorGeorgiou, I.en
dc.creatorVartholomatos, G.en
dc.creatorNtemou, A.en
dc.creatorFountzilas, Georgeen
dc.creatorPavlidis, Nicholasen
dc.date.accessioned2018-06-22T09:52:59Z
dc.date.available2018-06-22T09:52:59Z
dc.date.issued2008
dc.identifier.urihttps://gnosis.library.ucy.ac.cy/handle/7/41599
dc.description.abstractAims: In view of available targeted therapies, we investigated the presence of c-kit, PDGFR gene mutations and protein expression in cancer of unknown primary (CUP) in order to study their contribution in pathogenesis, their prognostic value and potential as therapeutic targets. Methods: Mutations in hot spots c-kit exon 11 and PDGFR exons 12 and 18 were studied in paraffin-embedded tumour samples from 50 patients with CUP by means of PCR-based single-strand conformational polymorphism and protein expression by means of streptavidin-biotin immunoperoxidase assays. Molecular markers were screened for possible correlations with patient outcome. Results: No shifted band was detected in any of the polyacrylamide gel electrophoreses, indicating absence of c-kit exon 11 and PDGFR exon 12, 18 mutations. Immunohistochemical analysis in 37 tumours revealed positive membranous CD117 expression in 30 samples (81%) of which five exhibited strong (+3), four moderate (+2) and 21 weak (+1) staining. PDGFRa protein staining was seen in 15 out of 30 (50%) cases, mostly weak (13) and rarely moderate (1) or strong (1). The expression of KIT or PDGFRa protein did not correlate with the clinical outcome of the patients in our cohort. Conclusions: In a moderate-sized CUP patient cohort, KIT or PDGFRa protein overexpression is rare, does not have gross prognostic significance for survival and is not associated with presence of activating mutations. © 2007 Springer-Verlag.en
dc.language.isoengen
dc.sourceJournal of cancer research and clinical oncologyen
dc.subjectArticleen
dc.subjectHumanen
dc.subjectNeoplasmsen
dc.subject80 and overen
dc.subjectAgeden
dc.subjectHumansen
dc.subjectAdulten
dc.subjectAgeden
dc.subjectCancer screeningen
dc.subjectFemaleen
dc.subjectMiddle ageden
dc.subjectPriority journalen
dc.subjectClinical articleen
dc.subjectHuman tissueen
dc.subjectPrognosisen
dc.subjectOutcome assessmenten
dc.subjectMaleen
dc.subjectPolymorphismen
dc.subjectExonen
dc.subjectGeneticen
dc.subjectPathogenesisen
dc.subjectCorrelation analysisen
dc.subjectProtein expressionen
dc.subjectImmunohistochemistryen
dc.subjectReceptorsen
dc.subjectCancer of unknown primary siteen
dc.subjectUnknown primaryen
dc.subjectImmunoperoxidase stainingen
dc.subjectIndolesen
dc.subjectExonsen
dc.subjectGene mutationen
dc.subjectPolyacrylamide gel electrophoresisen
dc.subjectCancer of unknown primaryen
dc.subjectGene targetingen
dc.subjectMutationen
dc.subjectPolymerase chain reactionen
dc.subjectSingle strand conformation polymorphismen
dc.subjectBiotinen
dc.subjectC-kiten
dc.subjectGastrointestinal stromal tumorsen
dc.subjectImmunoassayen
dc.subjectMolecular markeren
dc.subjectMutationsen
dc.subjectPdgfren
dc.subjectPeroxidaseen
dc.subjectPiperazinesen
dc.subjectPlatelet derived growth factor receptoren
dc.subjectPlatelet-derived growth factoren
dc.subjectProto-oncogene proteins c-kiten
dc.subjectPyrimidinesen
dc.subjectPyrrolesen
dc.subjectStem cell factor receptoren
dc.subjectStreptavidinen
dc.titleTargeting c-KIT, PDGFR in cancer of unknown primary: A screening study for molecular markers of benefiten
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1007/s00432-007-0341-7
dc.description.volume134
dc.description.issue6
dc.description.startingpage697
dc.description.endingpage704
dc.author.facultyΙατρική Σχολή / Medical School
dc.type.uhtypeArticleen


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