dc.contributor.author | Antoniadou, Zoi Anna | en |
dc.contributor.author | Hezka, Johana | en |
dc.contributor.author | Kousiappa, Ioanna | en |
dc.contributor.author | Mamais, Ioannis A. | en |
dc.contributor.author | Skoura, Lemonia G. | en |
dc.contributor.author | Pilalas, Dimitrios | en |
dc.contributor.author | Metallidis, Simeon A. | en |
dc.contributor.author | Nicolaidis, Pavlos | en |
dc.contributor.author | Malisiovas, Nikolaos | en |
dc.contributor.author | Kostrikis, Leontios G. | en |
dc.creator | Antoniadou, Zoi Anna | en |
dc.creator | Hezka, Johana | en |
dc.creator | Kousiappa, Ioanna | en |
dc.creator | Mamais, Ioannis A. | en |
dc.creator | Skoura, Lemonia G. | en |
dc.creator | Pilalas, Dimitrios | en |
dc.creator | Metallidis, Simeon A. | en |
dc.creator | Nicolaidis, Pavlos | en |
dc.creator | Malisiovas, Nikolaos | en |
dc.creator | Kostrikis, Leontios G. | en |
dc.date.accessioned | 2019-11-04T12:50:12Z | |
dc.date.available | 2019-11-04T12:50:12Z | |
dc.date.issued | 2014 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/52925 | |
dc.description.abstract | The emergence of resistance against current antiretroviral drugs to human immunodeficiency virus type 1 (HIV-1) is an increasingly important concern to the continuous success of antiretroviral therapy to HIV-1-infected patients. In the past decade, a number of studies reported that the prevalence of transmitted drug resistance among newly diagnosed patients has reached an overall 9% prevalence worldwide. Also, a number of studies using longitudinal HIV-1 patient study cohorts demonstrated that the cellular HIV-1 DNA level in peripheral blood mononuclear cells (PBMCs) has a prognostic value for the progression of HIV-1 disease independently of plasma HIV-1 RNA load and CD4 count. Using a previously established molecular-beacon-based real-time PCR methodology, cellular HIV-1 DNA levels were quantified in newly diagnosed and antiretroviral-naive patients in Northern Greece recruited between 2009 and 2010 using a predefined enrolling strategy, in an effort to investigate whether there is any relationship between cellular HIV-1 DNA levels and HIV-1 transmitted drug resistance. As part of the same study, DNA sequences encoding the env (C2-C5 region of gp120) were also amplified from PBMC-extracted DNA in order to determine the genotypic coreceptor tropism and genetic subtype. Cellular HIV-1 DNA levels had a median of 3.309 log10 HIV-1 copies per 106 PBMCs and demonstrated no correlation between cellular HIV-1 DNA levels and HIV-1 transmitted drug resistance. An absence of association between cellular HIV-1 DNA levels with plasma viral HIV-1 RNA load and CD4 levels was also found reconfirming the previously published study. Genotypic analysis of coreceptor tropism indicated that 96% of samples, independently of the presence or not of genotypic drug resistance, were CCR5-tropic. Overall, the findings reconfirmed the previously proposed proposition that transmitted drug resistance does not have an impact on disease progression in HIV-1-infected individuals. Also, CCR5 coreceptor tropism dominance suggests that both drug-resistant and drug-sensitive strains behave similarly in early infection in newly diagnosed patients. © Copyright 2014, Mary Ann Liebert, Inc. 2014. | en |
dc.source | AIDS Research and Human Retroviruses | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84895532050&doi=10.1089%2faid.2013.0160&partnerID=40&md5=936bb7f482cc6b49b4ed20efabb6b51b | |
dc.subject | Greece | en |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | adult | en |
dc.subject | controlled study | en |
dc.subject | female | en |
dc.subject | major clinical study | en |
dc.subject | priority journal | en |
dc.subject | prognosis | en |
dc.subject | disease course | en |
dc.subject | male | en |
dc.subject | genotype | en |
dc.subject | HIV Infections | en |
dc.subject | virus RNA | en |
dc.subject | Molecular Sequence Data | en |
dc.subject | Viral Load | en |
dc.subject | Human immunodeficiency virus 1 | en |
dc.subject | HIV-1 | en |
dc.subject | antiretrovirus agent | en |
dc.subject | CD4 lymphocyte count | en |
dc.subject | nucleotide sequence | en |
dc.subject | Human immunodeficiency virus 1 infection | en |
dc.subject | virus DNA | en |
dc.subject | DNA sequence | en |
dc.subject | real time polymerase chain reaction | en |
dc.subject | DNA, Viral | en |
dc.subject | Drug Resistance, Viral | en |
dc.subject | molecular beacon | en |
dc.subject | peripheral blood mononuclear cell | en |
dc.subject | Proviruses | en |
dc.subject | Sequence Analysis, DNA | en |
dc.subject | tropism | en |
dc.subject | Viral Tropism | en |
dc.title | Cellular HIV type 1 DNA levels are equivalent among drug-sensitive and drug-resistant strains in newly diagnosed and antiretroviral naive patients | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1089/aid.2013.0160 | |
dc.description.volume | 30 | |
dc.description.startingpage | 266 | |
dc.description.endingpage | 271 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :2</p> | en |
dc.source.abbreviation | AIDS Res.Hum.Retroviruses | en |
dc.contributor.orcid | Kostrikis, Leontios G. [0000-0002-5340-7109] | |
dc.gnosis.orcid | 0000-0002-5340-7109 | |