Nitrogen mustard up-regulates Bcl-2 and GSH and increases NTP and PCr in HT-29 colon cancer cells
dc.contributor.author | Boddie Jr., A. W. | en |
dc.contributor.author | Constantinou, Andreas I. | en |
dc.contributor.author | Williams, C. | en |
dc.contributor.author | Reed, A. | en |
dc.creator | Boddie Jr., A. W. | en |
dc.creator | Constantinou, Andreas I. | en |
dc.creator | Williams, C. | en |
dc.creator | Reed, A. | en |
dc.date.accessioned | 2019-11-04T12:50:16Z | |
dc.date.available | 2019-11-04T12:50:16Z | |
dc.date.issued | 1998 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/52954 | |
dc.description.abstract | We hypothesized that unexplained increases in nucleoside triphosphates (NTP) observed by 31P magnetic resonance spectroscopy (MRS) after treatment of tumours by DNA-damaging agents were related to chemotherapy-induced up-regulation of the bcl-2 gene and DNA damage prevention and repair processes. To test this hypothesis, we treated HT-29 cells with 10-4 M nitrogen mustard (HN2) and performed sequential perchloric acid extractions in replicate over 0-18 h. By reference to an internal standard (methylene diphosphonic acid), absolute changes in 31P-detectable high-energy phosphates in these extracts were determined and correlated with changes in bcl-2 protein levels, cell viability, cell cycle, apoptosis and total cellular glutathione (GSH) (an important defence against DNA damage from alkylating agents). After HN2 administration, bcl-2 protein levels in the HT-29 cell line rose at 2 h. Cell viability declined to 25% within 18 h, but apoptosis measured using fluorescence techniques remained in the 1-4% range. Increased cell division was noted at 4 h. Two high-energy interconvertible phosphates, NTP (P ≤ 0.006) and phosphocreatine (PCr) (P ≤ 0.0002), increased at 2 h concurrently with increased levels of bcl-2 protein and glutathione. This study demonstrates that bcl-2 and glutathione are up-regulated by HN2 and links this to a previously unexplained 31P MRS phenomenon: increased NTP after chemotherapy. | en |
dc.source | British journal of cancer | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-7144251162&partnerID=40&md5=f6a2cd0586472fa78efa4d0c204e8760 | |
dc.subject | article | en |
dc.subject | chlormethine | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | Chemotherapy | en |
dc.subject | priority journal | en |
dc.subject | protein bcl 2 | en |
dc.subject | human cell | en |
dc.subject | apoptosis | en |
dc.subject | Colonic Neoplasms | en |
dc.subject | cell cycle | en |
dc.subject | Up-Regulation | en |
dc.subject | Mechlorethamine | en |
dc.subject | Magnetic Resonance Spectroscopy | en |
dc.subject | fluorescence | en |
dc.subject | Ammonia | en |
dc.subject | cell viability | en |
dc.subject | Bcl-2 | en |
dc.subject | Adenosine Triphosphate | en |
dc.subject | Antineoplastic Agents, Alkylating | en |
dc.subject | ATP | en |
dc.subject | cell division | en |
dc.subject | cell strain ht29 | en |
dc.subject | Chromatography, High Pressure Liquid | en |
dc.subject | creatine phosphate | en |
dc.subject | Cytidine Triphosphate | en |
dc.subject | Genes, bcl-2 | en |
dc.subject | Glutathione | en |
dc.subject | Guanosine Triphosphate | en |
dc.subject | high energy phosphate | en |
dc.subject | HT29 Cells | en |
dc.subject | NTP | en |
dc.subject | nucleoside triphosphate | en |
dc.subject | Phosphocreatine | en |
dc.subject | Purine Nucleotides | en |
dc.subject | Uridine Triphosphate | en |
dc.title | Nitrogen mustard up-regulates Bcl-2 and GSH and increases NTP and PCr in HT-29 colon cancer cells | en |
dc.type | info:eu-repo/semantics/article | |
dc.description.volume | 77 | |
dc.description.startingpage | 1395 | |
dc.description.endingpage | 1404 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :8</p> | en |
dc.source.abbreviation | Br.J.Cancer | en |
dc.contributor.orcid | Constantinou, Andreas I. [0000-0003-0365-1821] | |
dc.gnosis.orcid | 0000-0003-0365-1821 |
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