Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo
dc.contributor.author | Charalambous, Anna | en |
dc.contributor.author | Andreou, Maria I. | en |
dc.contributor.author | Skourides, Paris A. | en |
dc.creator | Charalambous, Anna | en |
dc.creator | Andreou, Maria I. | en |
dc.creator | Skourides, Paris A. | en |
dc.date.accessioned | 2019-11-04T12:50:18Z | |
dc.date.available | 2019-11-04T12:50:18Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 1477-3155 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/52968 | |
dc.description.abstract | We describe an intein based method to site-specifically conjugate Quantum Dots (QDs) to target proteins in vivo. This approach allows the covalent conjugation of any nanostructure and/or nanodevice to any protein and thus the targeting of such material to any intracellular compartment or signalling complex within the cells of the developing embryo. We genetically fused a pleckstrin-homology (PH) domain with the N-terminus half of a split intein (IN). The C-terminus half (I) of the intein was conjugated to QDs in vitro. IC-QD's and RNA encoding PH-IN were microinjected into Xenopus embryos. In vivo intein-splicing resulted in fully functional QD-PH conjugates that could be monitored in real time within live embryos. Use of Near Infra Red (NIR)-emitting QDs allowed monitoring of QD-conjugates within the embryo at depths where EGFP is undetectable demonstrating the advantages of QD's for this type of experiment. In conclusion, we have developed a novel in vivo methodology for the site-specific conjugation of QD's and other artificial structures to target proteins in different intracellular compartments and signaling complexes. © 2009 Charalambous et al | en |
dc.description.abstract | licensee BioMed Central Ltd. | en |
dc.source | Journal of Nanobiotechnology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-74549213819&doi=10.1186%2f1477-3155-7-9&partnerID=40&md5=9addbd97aec11a90ca37144a401f17fb | |
dc.subject | Real time | en |
dc.subject | article | en |
dc.subject | amino acid sequence | en |
dc.subject | nonhuman | en |
dc.subject | protein targeting | en |
dc.subject | animal tissue | en |
dc.subject | RNA | en |
dc.subject | nanotechnology | en |
dc.subject | In-vitro | en |
dc.subject | In-vivo | en |
dc.subject | in vivo study | en |
dc.subject | Quantum dots | en |
dc.subject | Semiconductor quantum dots | en |
dc.subject | embryo | en |
dc.subject | protein domain | en |
dc.subject | Xenopus | en |
dc.subject | Proteins | en |
dc.subject | enhanced green fluorescent protein | en |
dc.subject | intein | en |
dc.subject | protein processing | en |
dc.subject | Artificial structures | en |
dc.subject | C-terminus | en |
dc.subject | conjugation | en |
dc.subject | Intracellular compartments | en |
dc.subject | Nano device | en |
dc.subject | Near infra red | en |
dc.subject | Pleckstrin homology | en |
dc.subject | Quantum Dot | en |
dc.subject | RNA encoding | en |
dc.subject | Signaling complex | en |
dc.subject | Signalling complex | en |
dc.subject | Site-specific | en |
dc.subject | Target proteins | en |
dc.subject | Xenopus embryo | en |
dc.title | Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1186/1477-3155-7-9 | |
dc.description.volume | 7 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :20</p> | en |
dc.source.abbreviation | J.Nanobiotechnology | en |
dc.contributor.orcid | Skourides, Paris A. [0000-0003-3502-5729] | |
dc.gnosis.orcid | 0000-0003-3502-5729 |
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