dc.contributor.author | Glittenberg, M. | en |
dc.contributor.author | Pitsouli, Chrysoula | en |
dc.contributor.author | Garvey, C. | en |
dc.contributor.author | Delidakis, Christos | en |
dc.contributor.author | Bray, S. | en |
dc.creator | Glittenberg, M. | en |
dc.creator | Pitsouli, Chrysoula | en |
dc.creator | Garvey, C. | en |
dc.creator | Delidakis, Christos | en |
dc.creator | Bray, S. | en |
dc.date.accessioned | 2019-11-04T12:50:39Z | |
dc.date.available | 2019-11-04T12:50:39Z | |
dc.date.issued | 2006 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53108 | |
dc.description.abstract | Notch is the receptor in a signalling pathway that operates in a diverse spectrum of developmental processes. Its ligands (e.g. Serrate) are transmembrane proteins whose signalling competence is regulated by the endocytosis-promoting E3 ubiquitin ligases, Mindbomb1 and Neuralized. The ligands also inhibit Notch present in the same cell (cis-inhibition). Here, we identify two conserved motifs in the intracellular domain of Serrate that are required for efficient endocytosis. The first, a dileucine motif, is dispensable for trans-activation and cis-inhibition despite the endocytic defect, demonstrating that signalling can be separated from bulk endocytosis. The second, a novel motif, is necessary for interactions with Mindbomb1/Neuralized and is strictly required for Serrate to trans-activate and internalise efficiently but not for it to inhibit Notch signalling. Cis-inhibition is compromised when an ER retention signal is added to Serrate, or when the levels of Neuralized are increased, and together these data indicate that cis-inhibitory interactions occur at the cell surface. The balance of ubiquitinated/unubiquitinated ligand will thus affect the signalling capacity of the cell at several levels. © 2006 European Molecular Biology Organization | All Rights Reserved. | en |
dc.source | EMBO Journal | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33750202549&doi=10.1038%2fsj.emboj.7601337&partnerID=40&md5=5d7d24181d755de4642e7c7398c5c206 | |
dc.subject | article | en |
dc.subject | controlled study | en |
dc.subject | priority journal | en |
dc.subject | unclassified drug | en |
dc.subject | nonhuman | en |
dc.subject | signal transduction | en |
dc.subject | Animals | en |
dc.subject | Calcium-Binding Proteins | en |
dc.subject | Intercellular Signaling Peptides and Proteins | en |
dc.subject | Jagged1 | en |
dc.subject | Membrane Proteins | en |
dc.subject | Notch | en |
dc.subject | membrane protein | en |
dc.subject | nucleotide sequence | en |
dc.subject | protein motif | en |
dc.subject | Cell Membrane | en |
dc.subject | Drosophila melanogaster | en |
dc.subject | protein domain | en |
dc.subject | protein protein interaction | en |
dc.subject | Protein Binding | en |
dc.subject | Drosophila | en |
dc.subject | Drosophila Proteins | en |
dc.subject | Protein Structure, Tertiary | en |
dc.subject | Protein Processing, Post-Translational | en |
dc.subject | transactivation | en |
dc.subject | Amino Acid Motifs | en |
dc.subject | cis isomer | en |
dc.subject | Endocytosis | en |
dc.subject | Endoplasmic Reticulum | en |
dc.subject | Mindbomb1 protein | en |
dc.subject | Neuralized protein | en |
dc.subject | Receptor, Notch1 | en |
dc.subject | Serrate | en |
dc.subject | Trans-Activation (Genetics) | en |
dc.subject | Transcriptional Activation | en |
dc.subject | Ubiquitin | en |
dc.subject | Ubiquitin-Protein Ligases | en |
dc.subject | Ubiquitination | en |
dc.title | Role of conserved intracellular motifs in Serrate signalling, cis-inhibition and endocytosis | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1038/sj.emboj.7601337 | |
dc.description.volume | 25 | |
dc.description.startingpage | 4697 | |
dc.description.endingpage | 4706 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :79</p> | en |
dc.source.abbreviation | EMBO J. | en |
dc.contributor.orcid | Pitsouli, Chrysoula [0000-0003-4074-9684] | |
dc.gnosis.orcid | 0000-0003-4074-9684 | |