dc.contributor.author | Salti, G. I. | en |
dc.contributor.author | Kichina, J. V. | en |
dc.contributor.author | Das Gupta, T. K. | en |
dc.contributor.author | Uddin, S. | en |
dc.contributor.author | Bratescu, L. | en |
dc.contributor.author | Pezzuto, J. M. | en |
dc.contributor.author | Mehta, R. G. | en |
dc.contributor.author | Constantinou, Andreas I. | en |
dc.creator | Salti, G. I. | en |
dc.creator | Kichina, J. V. | en |
dc.creator | Das Gupta, T. K. | en |
dc.creator | Uddin, S. | en |
dc.creator | Bratescu, L. | en |
dc.creator | Pezzuto, J. M. | en |
dc.creator | Mehta, R. G. | en |
dc.creator | Constantinou, Andreas I. | en |
dc.date.accessioned | 2019-11-04T12:52:35Z | |
dc.date.available | 2019-11-04T12:52:35Z | |
dc.date.issued | 2001 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53347 | |
dc.description.abstract | Melanoma transformation progresses in a multistep fashion from precursor lesions such as congenital naevi. Exposure to ultraviolet (UV) light promotes this process. Betulinic acid (BA) was identified by our group as a selective inhibitor of melanoma that functions by inducing apoptosis. The present study was designed to investigate the effect of BA and UV-C (254 nm) on cultured congenital melanocytic naevi (CMN) cells, using the single-cell gel electrophoresis (comet) assay to detect DNA damage. Exposure to UV light induced a 1.7-fold increase in CMN cells (P=0.008) when compared with controls. When a p53 genetic suppressor element that encodes a dominant negative polypeptide (termed GSE56) was introduced into the CMN cells, the transfected cells were more sensitive to UV-induced DNA breakage. This suggests that p53 can protect against UV-induced DNA damage and subsequent melanoma transformation. Pretreatment with BA (3 μm) for 48 h resulted in a 25.5% reduction in UV-induced DNA breakage in the CMN cells (P=0.023), but no changes were observed in the transfected cells. However, Western blot analysis revealed no changes in the p53 or p21 levels in BA-treated cells, suggesting that BA might mediate its action via a non-p53 pathway. These data indicate that BA may have an application as a chemopreventive agent in patients with congenital naevi. © 2001 Lippincott Williams & Wilkins. | en |
dc.source | Melanoma research | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035028281&doi=10.1097%2f00008390-200104000-00003&partnerID=40&md5=8920111c6cb4433c545ade93727935fc | |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | controlled study | en |
dc.subject | priority journal | en |
dc.subject | protein p53 | en |
dc.subject | Anticarcinogenic Agents | en |
dc.subject | cancer prevention | en |
dc.subject | human cell | en |
dc.subject | apoptosis | en |
dc.subject | DNA | en |
dc.subject | gel electrophoresis | en |
dc.subject | chemoprophylaxis | en |
dc.subject | malignant transformation | en |
dc.subject | Down-Regulation | en |
dc.subject | cell culture | en |
dc.subject | DNA strand breakage | en |
dc.subject | Tumor Cells, Cultured | en |
dc.subject | DNA Damage | en |
dc.subject | Blotting, Western | en |
dc.subject | Antineoplastic Agents, Phytogenic | en |
dc.subject | Genes, Dominant | en |
dc.subject | betulic acid | en |
dc.subject | Betulinic acid | en |
dc.subject | Comet Assay | en |
dc.subject | Congenital melanocytic naevi | en |
dc.subject | DNA damage score | en |
dc.subject | Genes, p53 | en |
dc.subject | Genetic suppressor element | en |
dc.subject | Melanocytes | en |
dc.subject | melanocytic nevus | en |
dc.subject | Neoplasms, Radiation-Induced | en |
dc.subject | Nevus | en |
dc.subject | rho GTP-Binding Proteins | en |
dc.subject | Triterpenes | en |
dc.subject | ultraviolet C radiation | en |
dc.subject | Ultraviolet Rays | en |
dc.title | Betulinic acid reduces ultraviolet-C-induced DNA breakage in congenital melanocytic naeval cells: Evidence for a potential role as a chemopreventive agent | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1097/00008390-200104000-00003 | |
dc.description.volume | 11 | |
dc.description.startingpage | 99 | |
dc.description.endingpage | 104 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Manufacturers: Indofine, United States | en |
dc.description.notes | Cited By :33</p> | en |
dc.source.abbreviation | Melanoma Res. | en |
dc.contributor.orcid | Constantinou, Andreas I. [0000-0003-0365-1821] | |
dc.gnosis.orcid | 0000-0003-0365-1821 | |