Polarized distribution of Bcr-Abl in migrating myeloid cells and co-localization of Bcr-Abl and its target proteins
dc.contributor.author | Skourides, Paris A. | en |
dc.contributor.author | Perera, S. A. | en |
dc.contributor.author | Ren, R. | en |
dc.creator | Skourides, Paris A. | en |
dc.creator | Perera, S. A. | en |
dc.creator | Ren, R. | en |
dc.date.accessioned | 2019-11-04T12:52:42Z | |
dc.date.available | 2019-11-04T12:52:42Z | |
dc.date.issued | 1999 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53393 | |
dc.description.abstract | Bcr-Abl plays a critical role in the pathogenesis of Philadelphia chromosome-positive leukemia. Although a large number of substrates and interacting proteins of Bcr-Abl have been identified, it remains unclear whether Bcr-Abl assembles multi-protein complexes and if it does where these complexes are within cells. We have investigated the localization of Bcr-Abl in 32D myeloid cells attached to the extracellular matrix. We have found that Bcr-Abl displays a polarized distribution, co-localizing with a subset of filamentous actin at trailing portions of migrating 32D cells, and localizes on the cortical F-actin and on vesicle-like structures in resting 32D cells. Deletion of the actin binding domain of Bcr-Abl (Bcr-Abl-AD) dramatically enhances the localization of Bcr-Abl on the vesicle-like structures. These distinct localization patterns of Bcr-Abl and Bcr-Abl-AD enabled us to examine the localization of Bcr-Abl substrate and interacting proteins in relation to Bcr-Abl. We found that a subset of biochemically defined target proteins of Bcr-Abl redistributed and co-localized with Bcr-Abl on F-actin and on vesicle-like structures. The co-localization of signaling proteins with Bcr-Abl at its sites of localization supports the idea that Bcr-Abl forms a multi-protein signaling complex, while the polarized distribution and vesicle-like localization of Bcr-Abl may play a role in leukemogenesis. | en |
dc.source | Oncogene | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033521922&doi=10.1038%2fsj.onc.1202407&partnerID=40&md5=e2ef3180c2b55b4ee05c160d808a3152 | |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | controlled study | en |
dc.subject | priority journal | en |
dc.subject | chronic myeloid leukemia | en |
dc.subject | signal transduction | en |
dc.subject | human cell | en |
dc.subject | Animals | en |
dc.subject | Mice | en |
dc.subject | Extracellular Matrix | en |
dc.subject | cell migration | en |
dc.subject | protein interaction | en |
dc.subject | bone marrow cell | en |
dc.subject | protein localization | en |
dc.subject | Proto-Oncogene Proteins | en |
dc.subject | Cell Adhesion | en |
dc.subject | Cell Movement | en |
dc.subject | Proteins | en |
dc.subject | Protein Binding | en |
dc.subject | Phosphorylation | en |
dc.subject | actin | en |
dc.subject | Actins | en |
dc.subject | Leukemia, Myeloid, Chronic | en |
dc.subject | Leukemia, Experimental | en |
dc.subject | Binding Sites | en |
dc.subject | Ubiquitin-Protein Ligases | en |
dc.subject | 3T3 Cells | en |
dc.subject | Adaptor Proteins, Signal Transducing | en |
dc.subject | Adaptor Proteins, Vesicular Transport | en |
dc.subject | bcr abl protein | en |
dc.subject | Bcr-Abl | en |
dc.subject | Cbl | en |
dc.subject | Cell Compartmentation | en |
dc.subject | Cell Polarity | en |
dc.subject | Chronic myelogenous leukemia | en |
dc.subject | f actin | en |
dc.subject | Fusion Proteins, bcr-abl | en |
dc.subject | Grb2 | en |
dc.subject | GRB2 Adaptor Protein | en |
dc.subject | Intracellular Membranes | en |
dc.subject | leukemogenesis | en |
dc.subject | Proto-Oncogene Proteins c-cbl | en |
dc.subject | Shc | en |
dc.title | Polarized distribution of Bcr-Abl in migrating myeloid cells and co-localization of Bcr-Abl and its target proteins | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1038/sj.onc.1202407 | |
dc.description.volume | 18 | |
dc.description.startingpage | 1165 | |
dc.description.endingpage | 1176 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :27</p> | en |
dc.source.abbreviation | Oncogene | en |
dc.contributor.orcid | Skourides, Paris A. [0000-0003-3502-5729] | |
dc.gnosis.orcid | 0000-0003-3502-5729 |
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