Persistent HIV-1 infection of natural killer cells in patients receiving highly active antiretroviral therapy
Patenaude, D. J.
Hatzakis, Angelos E.
Kostrikis, Leontios G.
Lazanas, Marios C.
Wyvill, K. M.
Pavlakis, G. N.
SourceProceedings of the National Academy of Sciences of the United States of America
Google Scholar check
MetadataShow full item record
We have identified a subset of CD56+CD3- human natural killer (NK) cells that express CD4 and the HIV coreceptors CCR5 and CXCR4. These cells can be productively infected in vitro by both CCR5- and CXCR4-using molecular clones of HIV-1 in a CD4-dependent manner. Analysis of HIV-infected persons showed that viral DNA is present in purified NK cells, and virus could be rescued from these cells after in vitro cultivation. Longitudinal analysis of the HIV-1 DNA levels in NK cells from patients after 1-2 years of highly active antiretroviral therapy indicated that NK cells remain persistently infected and account for a substantial amount of the viral DNA in peripheral blood mononuclear cells. These results demonstrate that a subset of non-T cells with NK markers are persistently infected and suggest that HIV infection of NK cells is important for virus persistence. The properties of the virus reservoir in these cells should be considered in attempts to further optimize antiretroviral therapies.
Showing items related by title, author, creator and subject.
Anastassopoulou, C. G.; Kostrikis, Leontios G. (2005)The transmission of HIV and the progression of HIV disease are influenced not only by a large number of human host factors, but also by certain correlates of the ever fluctuating virus quasispecies. The present review ...
Iacovides, D.; Michael, S.; Achilleos, Charis; Strati, K. (2013)A rise in technologies for epigenetic reprogramming of cells to pluripotency, highlights the potential of understanding and manipulating cellular plasticity in unprecedented ways. Increasing evidence points to shared ...
Re-analysis of human immunodeficiency virus type 1 isolates from Cyprus and Greece, initially designated 'subtype I', reveals a unique complex A/G/H/K/? mosaic pattern Paraskevis, Dimitrios N.; Magiorkinis, M.; Vandamme, A. M.; Kostrikis, Leontios G.; Hatzakis, Angelos E. (2001)Human immunodeficiency virus type 1 (HIV-1) has been classified into three main groups and 11 distinct subtypes. Moreover, several circulating recombinant forms (CRFs) of HIV-1 have been recently documented to have spread ...