Insights into the epithelial mesenchymal transition phenotype in cancer of unknown primary from a global microRNA profiling study
SourceClinical and Translational Oncology
Google Scholar check
MetadataShow full item record
Purpose: We sought to study the microRNA regulation of epithelial mesenchymal transition (EMT), the acquisition of migratory, mesenchymal-like properties of epithelial cells, in cancer of unknown primary (CUP). Patients and methods: We studied the global expression profile of 982 microRNAs by means of microarray technology in 68 CUP cases immunohistochemically characterised as EMT-positive (n = 5 by % of cells or n = 10 by a semiquantitative H-score) or EMT-negative. Results: EMT-suppressive miRNAs such as miR-203 and members of the miR-200 family (miR-200a,b,c and miR-141) presented a 2.45 to 3.64-fold lower expression level in the EMT-positive cases without, however, reaching statistical significance. MiR-205, a squamous tissue-specific marker, was very variable in the data set. Excluding CUP cases with squamous cell histology, miR-205, miR-203 and the miR-200 family exhibited a trend of downregulation in EMT-positive cases. A similar pattern of miRNA expression was detected when the comparison took place between EMT-positive vs EMT-negative cases according to the H-score. Moreover, miR-203, miR-205 and miR-200c were numerically downregulated in those tumours with high expression of the EMT marker N-cadherin. Conclusions: The EMT-suppressive miR-203 and miR-200 family were consistently but non-significantly downregulated in CUP with the EMT phenotype. A larger study is warranted to further explore the role of microRNAs in CUP. © 2013 Federación de Sociedades Españolas de Oncología (FESEO).
Showing items related by title, author, creator and subject.
Global microRNA profiling in favorable prognosis subgroups of cancer of unknown primary (CUP) demonstrates no significant expression differences with metastases of matched known primary tumors Pentheroudakis, George; Spector, Y.; Krikelis, D.; Kotoula, V.; Meiri, E.; Malamou-Mitsi, Vassiliki D.; Fountzilas, George; Sanden, M.; Pavlidis, Nicholas; Benjamin, H.; Aharonov, R. (2013)No data exist on biologic differences between Cancer of unknown primary (CUP) and metastatic solid tumors of known primary site. We assigned a primary tissue of origin in 40 favorable CUP patients (A: serous peritoneal ...
Kamposioras, K.; Pentheroudakis, George; Pavlidis, Nicholas (2013)Background: Cancer of unknown primary (CUP) ranks among the ten most common malignancies worldwide. Cancer of unknown primary presents as disseminated disease, has a dismal prognosis and remains a diagnosis of exclusion. ...
Novel microRNA-based assay demonstrates 92% agreement with diagnosis based on clinicopathologic and management data in a cohort of patients with carcinoma of unknown primary Pentheroudakis, George; Pavlidis, Nicholas; Fountzilas, George; Krikelis, D.; Goussia, Anna; Stoyianni, A.; Sanden, M.; Cyr, B. St; Yerushalmi, N.; Benjamin, H.; Meiri, E.; Chajut, A.; Rosenwald, S.; Aharonov, R.; Spector, Y. (2013)Background: Cancer of unknown or uncertain primary is a major diagnostic and clinical challenge, since identifying the tissue-of-origin of metastases is crucial for selecting optimal treatment. MicroRNAs are a family of ...