dc.contributor.author | Stylianopoulos, T. | en |
dc.contributor.author | Wong, C. | en |
dc.contributor.author | Bawendi, M. G. | en |
dc.contributor.author | Jain, R. K. | en |
dc.contributor.author | Fukumura, D. | en |
dc.creator | Stylianopoulos, T. | en |
dc.creator | Wong, C. | en |
dc.creator | Bawendi, M. G. | en |
dc.creator | Jain, R. K. | en |
dc.creator | Fukumura, D. | en |
dc.date.accessioned | 2019-05-06T12:24:41Z | |
dc.date.available | 2019-05-06T12:24:41Z | |
dc.date.issued | 2012 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/48869 | |
dc.description.abstract | The enhanced permeability and retention (EPR) effect has been a key rationale for the development of nanoscale carriers to solid tumors. As a consequence of EPR, nanotherapeutics are expected to improve drug and detection probe delivery, have less adverse effects than conventional chemotherapy, and thus result in improved detection and treatment of tumors. Physiological barriers posed by the abnormal tumor microenvironment, however, can hinder the homogeneous delivery of nanomedicine in amounts sufficient to eradicate cancer. To effectively enhance the therapeutic outcome of cancer patients by nanotherapeutics, we have to find ways to overcome these barriers. One possibility is to exploit the abnormal tumor microenvironment for selective and improved delivery of therapeutic agents to tumors. Recently, we proposed a multistage nanoparticle delivery system as a potential means to enable uniform delivery throughout the tumor and improve the efficacy of anticancer therapy. Here, we describe the synthesis of a novel multistage nanoparticle formulation that shrinks in size once it enters the tumor interstitial space to optimize the delivery to tumors as well as within tumors. Finally, we provide detailed experimental methods for the characterization of such nanoparticles. © 2012 Elsevier Inc. All rights reserved. | en |
dc.language.iso | eng | en |
dc.source | Methods in Enzymology | en |
dc.subject | article | en |
dc.subject | priority journal | en |
dc.subject | tumor microenvironment | en |
dc.subject | Solid tumors | en |
dc.subject | gelatinase A | en |
dc.subject | nonhuman | en |
dc.subject | cancer therapy | en |
dc.subject | drug half life | en |
dc.subject | Animals | en |
dc.subject | Mice | en |
dc.subject | Fluorescence | en |
dc.subject | in vitro study | en |
dc.subject | tumor | en |
dc.subject | Kinetics | en |
dc.subject | matrix metalloproteinase | en |
dc.subject | Blood | en |
dc.subject | drug delivery system | en |
dc.subject | drug distribution | en |
dc.subject | drug synthesis | en |
dc.subject | Drug delivery | en |
dc.subject | particle size | en |
dc.subject | nanoparticle | en |
dc.subject | collagen gel | en |
dc.subject | Nanoparticles | en |
dc.subject | drug penetration | en |
dc.subject | drug transport | en |
dc.subject | diffusion coefficient | en |
dc.subject | Nanomedicine | en |
dc.subject | Cancer nanotherapeutic | en |
dc.subject | circulation time | en |
dc.subject | drug degradation | en |
dc.subject | ECM (extracellular matrix) | en |
dc.subject | EPR (enhanced permeability and retention) | en |
dc.subject | fluorescence correlation spectroscopy | en |
dc.subject | gel filtration chromatography | en |
dc.subject | gelatin | en |
dc.subject | Intravital microscopy | en |
dc.subject | MMP2 (matrix metallopeptidase 2) | en |
dc.subject | quantum dot | en |
dc.subject | Quantum dots | en |
dc.subject | Spectrometry | en |
dc.subject | zymography | en |
dc.title | Multistage nanoparticles for improved delivery into tumor tissue | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1016/B978-0-12-391860-4.00006-9 | |
dc.description.volume | 508 | |
dc.description.startingpage | 109 | |
dc.description.endingpage | 130 | |
dc.author.faculty | Πολυτεχνική Σχολή / Faculty of Engineering | |
dc.author.department | Τμήμα Μηχανικών Μηχανολογίας και Κατασκευαστικής / Department of Mechanical and Manufacturing Engineering | |
dc.type.uhtype | Article | en |
dc.contributor.orcid | Stylianopoulos, T. [0000-0002-3093-1696] | |
dc.description.totalnumpages | 109-130 | |
dc.gnosis.orcid | 0000-0002-3093-1696 | |