KIF1Bβ transports dendritically localized mRNPs in neurons and is recruited to synapses in an activity-dependent manner
dc.contributor.author | Charalambous, Despina C. | en |
dc.contributor.author | Pasciuto, E. | en |
dc.contributor.author | Mercaldo, V. | en |
dc.contributor.author | Pilo Boyl, P. | en |
dc.contributor.author | Munck, S. | en |
dc.contributor.author | Bagni, C. | en |
dc.contributor.author | Santama, Niovi | en |
dc.creator | Charalambous, Despina C. | en |
dc.creator | Pasciuto, E. | en |
dc.creator | Mercaldo, V. | en |
dc.creator | Pilo Boyl, P. | en |
dc.creator | Munck, S. | en |
dc.creator | Bagni, C. | en |
dc.creator | Santama, Niovi | en |
dc.date.accessioned | 2019-11-04T12:50:19Z | |
dc.date.available | 2019-11-04T12:50:19Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1420-682X | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/52974 | |
dc.description.abstract | KIF1Bβ is a kinesin-like, microtubule-based molecular motor protein involved in anterograde axonal vesicular transport in vertebrate and invertebrate neurons. Certain KIF1Bβ isoforms have been implicated in different forms of human neurodegenerative disease, with characterization of their functional integration and regulation in the context of synaptic signaling still ongoing. Here, we characterize human KIF1Bβ (isoform NM015074), whose expression we show to be developmentally regulated and elevated in cortical areas of the CNS (including the motor cortex), in the hippocampus, and in spinal motor neurons. KIF1Bβ localizes to the cell body, axon, and dendrites, overlapping with synaptic-vesicle and postsynaptic-density structures. Correspondingly, in purified cortical synaptoneurosomes, KIF1Bβ is enriched in both pre- and postsynaptic structures, forming detergent-resistant complexes. Interestingly, KIF1Bβ forms RNA-protein complexes, containing the dendritically localized Arc and Calmodulin mRNAs, proteins previously shown to be part of RNA transport granules such as Purα, FMRP and FXR2P, and motor protein KIF3A, as well as Calmodulin. The interaction between KIF1Bβ and Calmodulin is Ca+2-dependent and takes place through a domain mapped at the carboxy-terminal tail of the motor. Live imaging of cortical neurons reveals active movement by KIF1Bβ at dendritic processes, suggesting that it mediates the transport of dendritically localized mRNAs. Finally, we show that synaptic recruitment of KIF1Bβ is activity-dependent and increased by stimulation of metabotropic or ionotropic glutamate receptors. The activity-dependent synaptic recruitment of KIF1Bβ, its interaction with Ca2+ sensor Calmodulin, and its new role as a dendritic motor of ribonucleoprotein complexes provide a novel basis for understanding the concerted co-ordination of motor protein mobilization and synaptic signaling pathways. © 2012 Springer Basel AG. | en |
dc.source | Cellular and Molecular Life Sciences | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84872359779&doi=10.1007%2fs00018-012-1108-0&partnerID=40&md5=30720a543fcc5d449ce8f84a3c5566b3 | |
dc.subject | article | en |
dc.subject | Humans | en |
dc.subject | controlled study | en |
dc.subject | protein expression | en |
dc.subject | unclassified drug | en |
dc.subject | nonhuman | en |
dc.subject | signal transduction | en |
dc.subject | Animals | en |
dc.subject | Mice | en |
dc.subject | animal cell | en |
dc.subject | mouse | en |
dc.subject | reverse transcription polymerase chain reaction | en |
dc.subject | nucleotide sequence | en |
dc.subject | protein localization | en |
dc.subject | Calcium | en |
dc.subject | Biological Transport | en |
dc.subject | Dendrites | en |
dc.subject | protein domain | en |
dc.subject | immunoprecipitation | en |
dc.subject | protein protein interaction | en |
dc.subject | central nervous system | en |
dc.subject | Vertebrata | en |
dc.subject | Invertebrata | en |
dc.subject | RNA, Messenger | en |
dc.subject | Arc | en |
dc.subject | Calmodulin | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | ionotropic receptor | en |
dc.subject | Kinesin | en |
dc.subject | kinesin family member 1B beta | en |
dc.subject | metabotropic receptor | en |
dc.subject | mGluR activation | en |
dc.subject | molecular motor | en |
dc.subject | Molecular Motor Proteins | en |
dc.subject | Molecular motors | en |
dc.subject | Motor Neurons | en |
dc.subject | mRNA transport | en |
dc.subject | Nerve Tissue Proteins | en |
dc.subject | Neurodegenerative Diseases | en |
dc.subject | plasticity | en |
dc.subject | postsynaptic density | en |
dc.subject | Protein Isoforms | en |
dc.subject | protein purification | en |
dc.subject | protein transport | en |
dc.subject | Receptors, Glutamate | en |
dc.subject | ribonucleoprotein | en |
dc.subject | Ribonucleoproteins | en |
dc.subject | RNA Interference | en |
dc.subject | RNA, Small Interfering | en |
dc.subject | synapse | en |
dc.subject | synapse vesicle | en |
dc.subject | Synaptic activity | en |
dc.subject | Synaptic Vesicles | en |
dc.title | KIF1Bβ transports dendritically localized mRNPs in neurons and is recruited to synapses in an activity-dependent manner | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1007/s00018-012-1108-0 | |
dc.description.volume | 70 | |
dc.description.startingpage | 335 | |
dc.description.endingpage | 356 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :14</p> | en |
dc.source.abbreviation | Cell Mol.Life Sci. | en |
dc.contributor.orcid | Santama, Niovi [0000-0002-8370-8674] | |
dc.gnosis.orcid | 0000-0002-8370-8674 |
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