dc.contributor.author | Constantinou, Andreas I. | en |
dc.contributor.author | Kiguchi, K. | en |
dc.contributor.author | Huberman, E. | en |
dc.creator | Constantinou, Andreas I. | en |
dc.creator | Kiguchi, K. | en |
dc.creator | Huberman, E. | en |
dc.date.accessioned | 2019-11-04T12:50:22Z | |
dc.date.available | 2019-11-04T12:50:22Z | |
dc.date.issued | 1990 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/52994 | |
dc.description.abstract | Genistein, an in vitro inhibitor of topoisomerase II and tyrosine kinases, suppressed growth and induced differentiation in HL-205 cells, a clonal population of the human promyelocytic HL-60 leukemia cells, and in K-562-J cells, a clonal population of the human erythroid K-562 leukemia cells. Maturing HL-205 cells acquired either granulocytic or monocytic markers, namely, reactivity with the murine OKM1 monoclonal antibody, expression of nitroblue tetrazolium dye reduction, and staining for nonspecific esterase. The maturing K-562-J cells stained with benzidine, which indicates the presence of hemoglobin, an erythroid maturation marker. Although the acquisition of the maturation markers in both HL-205 and K-562-J cells was time dependent up to 6 days, the kinetics of this induction differed between the two cell types. Despite the in vitro inhibitory effect of genistein, treatment of either HL-205 or K-562-J cells with 150 μg/ml genistein for up to 16 h did not alter topoisomerase II activity (as determined by the unknotting assay) in their nuclear extracts. Analysis with the anti-phosphotyrosine PY-20 murine monoclonal antibody indicated that treatment of K-562-J cells with genistein decreased the reactivity of the antibody with two of the cellular proteins. However, no reactivity with the PY-20 antibody was detected in untreated or genistein-treated HL-205 cells. An early event in the HL-205 and K-562-J cells, occurring after only 1 h of treatment with 30-200 μg/ml genistein, was the induction of DNA damage as measured by an alkaline elution assay. This damage may be a contributing factor in the genistein-induced cell differentiation in the HL-205 and K-562-J cells. © 1990, American Association for Cancer Research. All rights reserved. | en |
dc.source | Cancer research | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025363503&partnerID=40&md5=70cf3b214bb7300d8096d9121d592354 | |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | priority journal | en |
dc.subject | human cell | en |
dc.subject | DNA | en |
dc.subject | Leukemia | en |
dc.subject | cell differentiation | en |
dc.subject | Molecular Weight | en |
dc.subject | cell strain hl 60 | en |
dc.subject | DNA Topoisomerases, Type II | en |
dc.subject | Phosphorylation | en |
dc.subject | leukemia cell | en |
dc.subject | genistein | en |
dc.subject | Tumor Cells, Cultured | en |
dc.subject | Isoflavones | en |
dc.subject | dna topoisomerase (atp hydrolysing) | en |
dc.subject | Support, U.S. Gov't, Non-P.H.S. | en |
dc.subject | dna strand breakage | en |
dc.subject | DNA Damage | en |
dc.subject | cell strain k 562 | en |
dc.subject | Flavones | en |
dc.subject | Tyrosine | en |
dc.title | Induction of Differentiation and DNA Strand Breakage in Human HL-60 and K-562 Leukemia Cells by Genistein | en |
dc.type | info:eu-repo/semantics/article | |
dc.description.volume | 50 | |
dc.description.startingpage | 2618 | |
dc.description.endingpage | 2624 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Manufacturers: icn | en |
dc.description.notes | Cited By :229</p> | en |
dc.source.abbreviation | Cancer Res. | en |
dc.contributor.orcid | Constantinou, Andreas I. [0000-0003-0365-1821] | |
dc.gnosis.orcid | 0000-0003-0365-1821 | |