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dc.contributor.authorDubertret, B.en
dc.contributor.authorSkourides, Paris A.en
dc.contributor.authorNorris, D. J.en
dc.contributor.authorBrivanlou, A. H.en
dc.contributor.authorLibchaber, A.en
dc.contributor.authorNoireaux, V.fr
dc.creatorDubertret, B.en
dc.creatorSkourides, Paris A.en
dc.creatorNorris, D. J.en
dc.creatorBrivanlou, A. H.en
dc.creatorLibchaber, A.en
dc.creatorNoireaux, V.fr
dc.date.accessioned2019-11-04T12:50:31Z
dc.date.available2019-11-04T12:50:31Z
dc.date.issued2002
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/53056
dc.description.abstractFluorescent semiconductor nanocrystals (quantum dots) have the potential to revolutionize biological imaging, but their use has been limited by difficulties in obtaining nanocrystals that are biocompatible. To address this problem, we encapsulated individual nanocrystals in phospholipid block-copolymer micelles and demonstrated both in vitro and in vivo imaging. When conjugated to DNA, the nanocrystal-micelles acted as in vitro fluorescent probes to hybridize to specific complementary sequences. Moreover, when injected into Xenopus embryos, the nanocrystal-micelles were stable, nontoxic (<5 × 109 nanocrystals per cell), cell autonomous, and slow to photobleach. Nanocrystal fluorescence could be followed to the tadpole stage, allowing lineage-tracing experiments in embryogenesis.en
dc.sourceScienceen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-2242495402&doi=10.1126%2fscience.1077194&partnerID=40&md5=1979fffba17c5bdc8efa2c65bc5cb2e4
dc.subjectarticleen
dc.subjectpriority journalen
dc.subjectnonhumanen
dc.subjectPolyethylene Glycolsen
dc.subjectAnimalsen
dc.subjectEncapsulationen
dc.subjectDNAen
dc.subjectdiagnostic imagingen
dc.subjectcell interactionen
dc.subjectDNA sequenceen
dc.subjectMicellesen
dc.subjectCopolymersen
dc.subjectCrystallizationen
dc.subjectNanostructured materialsen
dc.subjectsemiconductoren
dc.subjectnanoparticleen
dc.subjectmolecular stabilityen
dc.subjectfluorescenceen
dc.subjectSemiconductor materialsen
dc.subjectnanotechnologyen
dc.subjectcopolymeren
dc.subjectBiological organsen
dc.subjectBiocompatibilityen
dc.subjectmicelleen
dc.subjectembryo developmenten
dc.subjectXenopusen
dc.subjectPhenotypeen
dc.subjectMicroinjectionsen
dc.subjectconjugationen
dc.subjectEmbryo, Nonmammalianen
dc.subjectOligodeoxyribonucleotidesen
dc.subjectEmbryonic Developmenten
dc.subject1,2-Dipalmitoylphosphatidylcholineen
dc.subjectcomplementary DNAen
dc.subjectComplementary sequencesen
dc.subjectDNA hybridizationen
dc.subjectDNA structureen
dc.subjectfluorescent dyeen
dc.subjectFluorescent Dyesen
dc.subjectimaging methoden
dc.subjectMicroscopy, Electronen
dc.subjectMicroscopy, Fluorescenceen
dc.subjectNucleic Acid Hybridizationen
dc.subjectPhosphatidylethanolaminesen
dc.subjectphospholipiden
dc.subjectPhospholipidsen
dc.subjectquantum chemistryen
dc.subjectSemiconductorsen
dc.subjecttadpoleen
dc.titleIn vivo imaging of quantum dots encapsulated in phospholipid micellesen
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1126/science.1077194
dc.description.volume298
dc.description.startingpage1759
dc.description.endingpage1762
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeArticleen
dc.source.abbreviationScienceen
dc.contributor.orcidSkourides, Paris A. [0000-0003-3502-5729]
dc.gnosis.orcid0000-0003-3502-5729


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