dc.contributor.author | Ganapathi, R. | en |
dc.contributor.author | Zwelling, L. | en |
dc.contributor.author | Constantinou, Andreas I. | en |
dc.contributor.author | Ford, J. | en |
dc.contributor.author | Grabowski, D. | en |
dc.creator | Ganapathi, R. | en |
dc.creator | Zwelling, L. | en |
dc.creator | Constantinou, Andreas I. | en |
dc.creator | Ford, J. | en |
dc.creator | Grabowski, D. | en |
dc.date.accessioned | 2019-11-04T12:50:35Z | |
dc.date.available | 2019-11-04T12:50:35Z | |
dc.date.issued | 1993 | |
dc.identifier.issn | 0006-291X | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53081 | |
dc.description.abstract | Resistance to amsacrine in HL-60/AMSA is 50-100 fold compared to the parental HL-60/S cells. Synthesis and phosphorylation of topoisomerase II (TOPO II) were 2-3 fold lower in HL-60/AMSA compared to HL-60/S cells metabolically labelled with [32P]-orthophosphoric acid or [35S]-L-methionine. Incubating cells in radiolabel-free media following metabolic labelling for 4 hr revealed: (a) dephosphorylation of topoisomerase II at 4 hr was 70% and 20% in HL-60/S and HL-60/AMSA cells, respectively | en |
dc.description.abstract | and (b) degradation of topoisomerase II at 4 hr was 40% and 10% in HL-60/S and HL-60/AMSA cells, respectively, while at 8 hr degradation was 80% and 50% in HL-60/S and HL60/AMSA cells, respectively. The magnitude of topoisomerase II band depletion in immunoprecipitates of amsacrine-treated cells labelled with [35S}-L-methionine or [32P]-orthophosphoric acid, correlated with the differential amsacrine sensitivity of HL-60/S and HL-60/AMSA cells, suggesting that the amount of newly synthesized and phosphorylated topoisomerase II may be contributing to amsacrine resistance. Thus, the attenuated synthesis and phosphorylation of TOPO II in HL-60/AMSA may contribute to the resistance of these cells to amsacrine. © 1993 Academic Press, Inc. | en |
dc.source | Biochemical and biophysical research communications | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027170607&doi=10.1006%2fbbrc.1993.1554&partnerID=40&md5=79cf584cf3ca62cdad1166a8a9264a18 | |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | priority journal | en |
dc.subject | Drug Resistance | en |
dc.subject | human cell | en |
dc.subject | biosynthesis | en |
dc.subject | Kinetics | en |
dc.subject | Molecular Weight | en |
dc.subject | phosphorylation | en |
dc.subject | Support, Non-U.S. Gov't | en |
dc.subject | cell culture | en |
dc.subject | immunoprecipitation | en |
dc.subject | amsacrine | en |
dc.subject | DNA Topoisomerases, Type II | en |
dc.subject | Isoenzymes | en |
dc.subject | Support, U.S. Gov't, P.H.S. | en |
dc.subject | leukemia cell | en |
dc.subject | Tumor Cells, Cultured | en |
dc.subject | Leukemia, Promyelocytic, Acute | en |
dc.subject | dna topoisomerase | en |
dc.subject | Electrophoresis, Polyacrylamide Gel | en |
dc.subject | Autoradiography | en |
dc.subject | methionine | en |
dc.subject | Phosphates | en |
dc.subject | Phosphorus Radioisotopes | en |
dc.subject | Sulfur Radioisotopes | en |
dc.title | Altered Phosphorylation, Biosynthesis and Degradation of the 170 kDa Isoform of Topoisomerase II in Amsacrine-Resistant Human Leukemia Cells | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1006/bbrc.1993.1554 | |
dc.description.volume | 192 | |
dc.description.startingpage | 1274 | |
dc.description.endingpage | 1280 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :20</p> | en |
dc.source.abbreviation | Biochem.Biophys.Res.Commun. | en |
dc.contributor.orcid | Constantinou, Andreas I. [0000-0003-0365-1821] | |
dc.gnosis.orcid | 0000-0003-0365-1821 | |