The pathogenic properties of a novel and conserved gene product, KerV, in proteobacteria
dc.contributor.author | An, D. | en |
dc.contributor.author | Apidianakis, Yiorgos | en |
dc.contributor.author | Boechat, A. L. | en |
dc.contributor.author | Baldini, R. L. | en |
dc.contributor.author | Goumnerov, B. C. | en |
dc.contributor.author | Rahme, L. G. | en |
dc.creator | An, D. | en |
dc.creator | Apidianakis, Yiorgos | en |
dc.creator | Boechat, A. L. | en |
dc.creator | Baldini, R. L. | en |
dc.creator | Goumnerov, B. C. | en |
dc.creator | Rahme, L. G. | en |
dc.date.accessioned | 2019-11-04T12:50:10Z | |
dc.date.available | 2019-11-04T12:50:10Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/52913 | |
dc.description.abstract | Identification of novel virulence factors is essential for understanding bacterial pathogenesis and designing antibacterial strategies. In this study, we uncover such a factor, termed KerV, in Proteobacteria. Experiments carried out in a variety of eukaryotic host infection models revealed that the virulence of a Pseudomonas aeruginosa kerV null mutant was compromised when it interacted with amoebae, plants, flies, and mice. Bioinformatics analyses indicated that KerV is a hypothetical methyltransferase and is well-conserved across numerous Proteobacteria, including both well-known and emerging pathogens (e.g., virulent Burkholderia, Escherichia, Shigella, Vibrio, Salmonella, Yersinia and Brucella species). Furthermore, among the 197 kerV orthologs analyzed in this study, about 89% reside in a defined genomic neighborhood, which also possesses essential DNA replication and repair genes and detoxification gene. Finally, infection of Drosophila melanogaster with null mutants demonstrated that KerV orthologs are also crucial in Vibrio cholerae and Yersinia pseudotuberculosis pathogenesis. Our findings suggested that KerV has a novel and broad significance as a virulence factor in pathogenic Proteobacteria and it might serve as a new target for antibiotic drug design. © 2009 An et al. | en |
dc.source | PLoS ONE | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349686687&doi=10.1371%2fjournal.pone.0007167&partnerID=40&md5=5b1d7521f9173b469c3cbf58247f7a0e | |
dc.subject | methodology | en |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | controlled study | en |
dc.subject | female | en |
dc.subject | pathogenesis | en |
dc.subject | Computational Biology | en |
dc.subject | gene product | en |
dc.subject | unclassified drug | en |
dc.subject | gene expression regulation | en |
dc.subject | nonhuman | en |
dc.subject | metabolism | en |
dc.subject | Animals | en |
dc.subject | Anti-Bacterial Agents | en |
dc.subject | Mice | en |
dc.subject | animal | en |
dc.subject | animal experiment | en |
dc.subject | animal model | en |
dc.subject | mouse | en |
dc.subject | genetics | en |
dc.subject | microbiology | en |
dc.subject | antiinfective agent | en |
dc.subject | physiology | en |
dc.subject | gene mutation | en |
dc.subject | phylogeny | en |
dc.subject | genetic analysis | en |
dc.subject | Pseudomonas aeruginosa | en |
dc.subject | nucleotide sequence | en |
dc.subject | phylogenetic tree | en |
dc.subject | biology | en |
dc.subject | Arabidopsis | en |
dc.subject | Bacteria (microorganisms) | en |
dc.subject | bacterial protein | en |
dc.subject | Bacterial Proteins | en |
dc.subject | bacterial virulence | en |
dc.subject | bioinformatics | en |
dc.subject | Brucella | en |
dc.subject | Burkholderia | en |
dc.subject | Conserved Sequence | en |
dc.subject | DNA repair | en |
dc.subject | DNA replication | en |
dc.subject | Drosophila melanogaster | en |
dc.subject | Escherichia | en |
dc.subject | Eukaryota | en |
dc.subject | eukaryotic cell | en |
dc.subject | Gene Expression Regulation, Bacterial | en |
dc.subject | gene interaction | en |
dc.subject | genetic conservation | en |
dc.subject | methyltransferase | en |
dc.subject | Methyltransferases | en |
dc.subject | Mus | en |
dc.subject | orthology | en |
dc.subject | protein KerV | en |
dc.subject | Proteobacteria | en |
dc.subject | Salmonella | en |
dc.subject | Shigella | en |
dc.subject | Vibrio | en |
dc.subject | Vibrio cholerae | en |
dc.subject | virulence factor | en |
dc.subject | Virulence Factors | en |
dc.subject | Yersinia | en |
dc.subject | Yersinia pseudotuberculosis | en |
dc.title | The pathogenic properties of a novel and conserved gene product, KerV, in proteobacteria | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1371/journal.pone.0007167 | |
dc.description.volume | 4 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :5</p> | en |
dc.source.abbreviation | PLoS ONE | en |
dc.contributor.orcid | Apidianakis, Yiorgos [0000-0002-7465-3560] | |
dc.gnosis.orcid | 0000-0002-7465-3560 |
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