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dc.contributor.authorAn, D.en
dc.contributor.authorApidianakis, Yiorgosen
dc.contributor.authorBoechat, A. L.en
dc.contributor.authorBaldini, R. L.en
dc.contributor.authorGoumnerov, B. C.en
dc.contributor.authorRahme, L. G.en
dc.creatorAn, D.en
dc.creatorApidianakis, Yiorgosen
dc.creatorBoechat, A. L.en
dc.creatorBaldini, R. L.en
dc.creatorGoumnerov, B. C.en
dc.creatorRahme, L. G.en
dc.date.accessioned2019-11-04T12:50:10Z
dc.date.available2019-11-04T12:50:10Z
dc.date.issued2009
dc.identifier.issn1932-6203
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/52913
dc.description.abstractIdentification of novel virulence factors is essential for understanding bacterial pathogenesis and designing antibacterial strategies. In this study, we uncover such a factor, termed KerV, in Proteobacteria. Experiments carried out in a variety of eukaryotic host infection models revealed that the virulence of a Pseudomonas aeruginosa kerV null mutant was compromised when it interacted with amoebae, plants, flies, and mice. Bioinformatics analyses indicated that KerV is a hypothetical methyltransferase and is well-conserved across numerous Proteobacteria, including both well-known and emerging pathogens (e.g., virulent Burkholderia, Escherichia, Shigella, Vibrio, Salmonella, Yersinia and Brucella species). Furthermore, among the 197 kerV orthologs analyzed in this study, about 89% reside in a defined genomic neighborhood, which also possesses essential DNA replication and repair genes and detoxification gene. Finally, infection of Drosophila melanogaster with null mutants demonstrated that KerV orthologs are also crucial in Vibrio cholerae and Yersinia pseudotuberculosis pathogenesis. Our findings suggested that KerV has a novel and broad significance as a virulence factor in pathogenic Proteobacteria and it might serve as a new target for antibiotic drug design. © 2009 An et al.en
dc.sourcePLoS ONEen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-70349686687&doi=10.1371%2fjournal.pone.0007167&partnerID=40&md5=5b1d7521f9173b469c3cbf58247f7a0e
dc.subjectmethodologyen
dc.subjectarticleen
dc.subjecthumanen
dc.subjectHumansen
dc.subjectcontrolled studyen
dc.subjectfemaleen
dc.subjectpathogenesisen
dc.subjectComputational Biologyen
dc.subjectgene producten
dc.subjectunclassified drugen
dc.subjectgene expression regulationen
dc.subjectnonhumanen
dc.subjectmetabolismen
dc.subjectAnimalsen
dc.subjectAnti-Bacterial Agentsen
dc.subjectMiceen
dc.subjectanimalen
dc.subjectanimal experimenten
dc.subjectanimal modelen
dc.subjectmouseen
dc.subjectgeneticsen
dc.subjectmicrobiologyen
dc.subjectantiinfective agenten
dc.subjectphysiologyen
dc.subjectgene mutationen
dc.subjectphylogenyen
dc.subjectgenetic analysisen
dc.subjectPseudomonas aeruginosaen
dc.subjectnucleotide sequenceen
dc.subjectphylogenetic treeen
dc.subjectbiologyen
dc.subjectArabidopsisen
dc.subjectBacteria (microorganisms)en
dc.subjectbacterial proteinen
dc.subjectBacterial Proteinsen
dc.subjectbacterial virulenceen
dc.subjectbioinformaticsen
dc.subjectBrucellaen
dc.subjectBurkholderiaen
dc.subjectConserved Sequenceen
dc.subjectDNA repairen
dc.subjectDNA replicationen
dc.subjectDrosophila melanogasteren
dc.subjectEscherichiaen
dc.subjectEukaryotaen
dc.subjecteukaryotic cellen
dc.subjectGene Expression Regulation, Bacterialen
dc.subjectgene interactionen
dc.subjectgenetic conservationen
dc.subjectmethyltransferaseen
dc.subjectMethyltransferasesen
dc.subjectMusen
dc.subjectorthologyen
dc.subjectprotein KerVen
dc.subjectProteobacteriaen
dc.subjectSalmonellaen
dc.subjectShigellaen
dc.subjectVibrioen
dc.subjectVibrio choleraeen
dc.subjectvirulence factoren
dc.subjectVirulence Factorsen
dc.subjectYersiniaen
dc.subjectYersinia pseudotuberculosisen
dc.titleThe pathogenic properties of a novel and conserved gene product, KerV, in proteobacteriaen
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1371/journal.pone.0007167
dc.description.volume4
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeArticleen
dc.description.notes<p>Cited By :5</p>en
dc.source.abbreviationPLoS ONEen
dc.contributor.orcidApidianakis, Yiorgos [0000-0002-7465-3560]
dc.gnosis.orcid0000-0002-7465-3560


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