dc.contributor.author | Andreou, Maria I. | en |
dc.contributor.author | Yan, C. Y. I. | en |
dc.contributor.author | Skourides, Paris A. | en |
dc.creator | Andreou, Maria I. | en |
dc.creator | Yan, C. Y. I. | en |
dc.creator | Skourides, Paris A. | en |
dc.date.accessioned | 2019-11-04T12:50:11Z | |
dc.date.available | 2019-11-04T12:50:11Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/52917 | |
dc.description.abstract | Heterogeneous nuclear ribonucleoproteins (hnRNPs) comprise a large group of modular RNA-binding proteins classified according to their conserved domains. This modular nature, coupled with a large choice of alternative splice variants generates functional diversity. Here, we investigate the biological differences between 40LoVe, its splice variant Samba and its pseudoallele hnRNP AB in neural development. Loss of function experiments lead to defects in neural development with reduction of eye size, which stem primarily from increased apoptosis and reduced proliferation in neural tissues. Despite very high homology between 40LoVe/Samba and hnRNP AB, these proteins display major differences in localization, which appear to be in part responsible for functional differences. Specifically, we show that the 40Love/Samba carboxy-terminal domain (GRD) enables nucleocytoplasmic shuttling behavior. This domain is slightly different in hnRNP AB, leading to nuclear-restricted localization. Finally, we show that shuttling is required for 40LoVe/Samba function in neural development. © 2014 Andreou et al. | en |
dc.source | PLoS ONE | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84898604694&doi=10.1371%2fjournal.pone.0085026&partnerID=40&md5=879528ae62e8c58915178e3f4e3f0013 | |
dc.subject | article | en |
dc.subject | controlled study | en |
dc.subject | amino acid sequence | en |
dc.subject | cell proliferation | en |
dc.subject | unclassified drug | en |
dc.subject | nonhuman | en |
dc.subject | metabolism | en |
dc.subject | molecular genetics | en |
dc.subject | apoptosis | en |
dc.subject | Animals | en |
dc.subject | animal | en |
dc.subject | In Situ Nick-End Labeling | en |
dc.subject | nick end labeling | en |
dc.subject | physiology | en |
dc.subject | Molecular Sequence Data | en |
dc.subject | RNA splicing | en |
dc.subject | reverse transcription polymerase chain reaction | en |
dc.subject | protein function | en |
dc.subject | chemistry | en |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
dc.subject | protein localization | en |
dc.subject | in situ hybridization | en |
dc.subject | nervous system | en |
dc.subject | 40Love protein | en |
dc.subject | 40LoVe protein, Xenopus | en |
dc.subject | carboxy terminal sequence | en |
dc.subject | embryo | en |
dc.subject | embryo development | en |
dc.subject | growth, development and aging | en |
dc.subject | heterogeneous nuclear ribonucleoprotein | en |
dc.subject | Heterogeneous-Nuclear Ribonucleoprotein Group A-B | en |
dc.subject | Heterogeneous-Nuclear Ribonucleoproteins | en |
dc.subject | nerve cell differentiation | en |
dc.subject | nervous tissue | en |
dc.subject | nucleocytoplasmic transport | en |
dc.subject | protein domain | en |
dc.subject | RNA binding protein | en |
dc.subject | Samba protein | en |
dc.subject | samba protein, Xenopus | en |
dc.subject | sequence homology | en |
dc.subject | Sequence Homology, Amino Acid | en |
dc.subject | Xenopus | en |
dc.subject | Xenopus protein | en |
dc.subject | Xenopus Proteins | en |
dc.title | 40LoVe and Samba are involved in Xenopusneural development and functionally distinct from hnRNP AB | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1371/journal.pone.0085026 | |
dc.description.volume | 9 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.source.abbreviation | PLoS ONE | en |
dc.contributor.orcid | Skourides, Paris A. [0000-0003-3502-5729] | |
dc.gnosis.orcid | 0000-0003-3502-5729 | |