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dc.contributor.authorConstantinou, Constantinaen
dc.contributor.authorApidianakis, Yiorgosen
dc.contributor.authorPsychogios, N.en
dc.contributor.authorRighi, V.en
dc.contributor.authorMindrinos, M. N.en
dc.contributor.authorKhan, N.en
dc.contributor.authorSwartz, H. M.en
dc.contributor.authorSzeto, H. H.en
dc.contributor.authorTompkins, R. G.en
dc.contributor.authorRahme, L. G.en
dc.contributor.authorAria Tzika, A.en
dc.creatorConstantinou, Constantinaen
dc.creatorApidianakis, Yiorgosen
dc.creatorPsychogios, N.en
dc.creatorRighi, V.en
dc.creatorMindrinos, M. N.en
dc.creatorKhan, N.en
dc.creatorSwartz, H. M.en
dc.creatorSzeto, H. H.en
dc.creatorTompkins, R. G.en
dc.creatorRahme, L. G.en
dc.creatorAria Tzika, A.en
dc.date.accessioned2019-11-04T12:50:24Z
dc.date.available2019-11-04T12:50:24Z
dc.date.issued2016
dc.identifier.issn1107-3756
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/53008
dc.description.abstractTrauma is the most common cause of mortality among individuals aged between 1 and 44 years and the third leading cause of mortality overall in the US. In this study, we examined the effects of trauma on the expression of genes in Drosophila melanogaster, a useful model for investigating genetics and physiology. After trauma was induced by a non-lethal needle puncture of the thorax, we observed the differential expression of genes encoding for mitochondrial uncoupling proteins, as well as those encoding for apoptosis-related and insulin signaling-related proteins, thus indicating muscle functional dysregulation. These results prompted us to examine the link between insulin signaling and mitochondrial dysfunction using in vivo nuclear magnetic resonance (NMR) with complementary electron paramagnetic resonance (EPR) spectroscopy. Trauma significantly increased insulin resistance biomarkers, and the NMR spectral profile of the aged flies with trauma-induced thoracic injury resembled that of insulin-resistant chico mutant flies. In addition, the mitochondrial redox status, as measured by EPR, was significantly altered following trauma, indicating mitochondrial uncoupling. A mitochondria-targeted compound, Szeto-Schiller (SS)-31 that promotes adenosine triphosphate (ATP) synthesis normalized the NMR spectral profile, as well as the mitochondrial redox status of the flies with trauma-induced thoracic injury, as assessed by EPR. Based on these findings, we propose a molecular mechanism responsible for trauma-related mortality and also propose that trauma sequelae in aging are linked to insulin signaling and mitochondrial dysfunction. Our findings further suggest that SS-31 attenuates trauma-associated pathological changes.en
dc.sourceInternational journal of molecular medicineen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84954544853&doi=10.3892%2fijmm.2015.2426&partnerID=40&md5=601cc482f56420ee8ffef14462d1faf0
dc.subjecthumanen
dc.subjectHumansen
dc.subjectageden
dc.subjectcontrolled studyen
dc.subjectpriority journalen
dc.subjectAgingen
dc.subjectmaleen
dc.subjectunclassified drugen
dc.subjectcomplicationen
dc.subjectnonhumanen
dc.subjectpathologyen
dc.subjectsignal transductionen
dc.subjectupregulationen
dc.subjectArticleen
dc.subjectmetabolismen
dc.subjectbiological markeren
dc.subjectgene expressionen
dc.subjectapoptosisen
dc.subjectAnimalsen
dc.subjectanimalen
dc.subjectanimal experimenten
dc.subjectanimal modelen
dc.subjectbiosynthesisen
dc.subjectdisease modelen
dc.subjectgeneticsen
dc.subjectinjuryen
dc.subjectimage analysisen
dc.subjectOligopeptidesen
dc.subjectBiomarkersen
dc.subjectWounds and Injuriesen
dc.subjectthorax injuryen
dc.subjectmedical schoolen
dc.subjectMagnetic Resonance Spectroscopyen
dc.subjectnuclear magnetic resonance spectroscopyen
dc.subjectelectron spin resonanceen
dc.subjectElectron paramagnetic resonanceen
dc.subjectNuclear magnetic resonanceen
dc.subjectin vivo studyen
dc.subjectDrosophila melanogasteren
dc.subjectDisease Models, Animalen
dc.subjectinsulin receptoren
dc.subjectIon Channelsen
dc.subjectadenosine triphosphateen
dc.subjectapoptosis inducing factoren
dc.subjectarginyl-2,'6'-dimethyltyrosyl-lysyl-phenylalaninamideen
dc.subjectDrosophila forkhead box O proteinen
dc.subjectDrosophila phosphatase and tensin homolog proteinen
dc.subjectElectron Spin Resonance Spectroscopyen
dc.subjectenzyme synthesisen
dc.subjectgenome analysisen
dc.subjectHigh-resolution magic angle spinningen
dc.subjectinsulin resistanceen
dc.subjectInsulin signalingen
dc.subjection channelen
dc.subjectMitochondriaen
dc.subjectmitochondrial proteinen
dc.subjectMitochondrial Proteinsen
dc.subjectmitochondrial uncoupling protein 4en
dc.subjectmitochondrionen
dc.subjectoligopeptideen
dc.subjectoxidative phosphorylation uncouplingen
dc.subjectThoracic Injuriesen
dc.subjectuncoupling protein 1en
dc.titleIn vivo high-resolution magic angle spinning magnetic and electron paramagnetic resonance spectroscopic analysis of mitochondria-targeted peptide in Drosophila melanogaster with trauma-induced thoracic injuryen
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.3892/ijmm.2015.2426
dc.description.volume37
dc.description.startingpage299
dc.description.endingpage308
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeArticleen
dc.source.abbreviationInt.J.Mol.Med.en
dc.contributor.orcidApidianakis, Yiorgos [0000-0002-7465-3560]
dc.gnosis.orcid0000-0002-7465-3560


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