dc.contributor.author | Gentile, M. S. | en |
dc.contributor.author | Vasu, C. | en |
dc.contributor.author | Green, A. | en |
dc.contributor.author | Murillo, G. | en |
dc.contributor.author | Das Gupta, T. K. | en |
dc.contributor.author | Constantinou, Andreas I. | en |
dc.contributor.author | Prabhakar, B. S. | en |
dc.contributor.author | Salti, G. I. | en |
dc.creator | Gentile, M. S. | en |
dc.creator | Vasu, C. | en |
dc.creator | Green, A. | en |
dc.creator | Murillo, G. | en |
dc.creator | Das Gupta, T. K. | en |
dc.creator | Constantinou, Andreas I. | en |
dc.creator | Prabhakar, B. S. | en |
dc.creator | Salti, G. I. | en |
dc.date.accessioned | 2019-11-04T12:50:36Z | |
dc.date.available | 2019-11-04T12:50:36Z | |
dc.date.issued | 2003 | |
dc.identifier.issn | 1019-6439 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53084 | |
dc.description.abstract | We have shown that genistein, a major component of soy, has anti-colon cancer effects in vitro. These effects are attainable at high concentrations that are difficult to achieve in the serum. The purpose of this study was to enhance the activity of genistein against colon cancer cells by coupling it to 17.1A. The monoclonal antibody 17.1A recognizes an epithelial membrane antigen that is overexpressed in colon cancer. Synthesis of Gen-17.1A was achieved by photochemical conjugation using sulfa-SANPAH. Its purity was evaluated by SDS-PAGE. Binding of Gen-17.1A to SW-620 and HT-29 cells was shown using flow cytometry. Internalization was demonstrated by FITC-labeling. Gen-17.1A induced apoptosis in colon cancer cells as evidenced by the acridine orange/ethidium bromide staining method. Gen-17.1A significantly inhibited colon cancer cell growth in vitro and in vivo. Our findings suggest that conjugating genistein to 17.1A monoclonal antibody enhances its effects against colon cancer cells. | en |
dc.source | International journal of oncology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042128695&partnerID=40&md5=127dfe46c9634eb800f9cba3de4ff8e8 | |
dc.subject | article | en |
dc.subject | antineoplastic agent | en |
dc.subject | Antineoplastic Agents | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | drug effect | en |
dc.subject | monoclonal antibody | en |
dc.subject | pathology | en |
dc.subject | drug derivative | en |
dc.subject | metabolism | en |
dc.subject | apoptosis | en |
dc.subject | Animals | en |
dc.subject | Mice | en |
dc.subject | animal | en |
dc.subject | mouse | en |
dc.subject | Colonic Neoplasms | en |
dc.subject | cell membrane | en |
dc.subject | Biological Transport | en |
dc.subject | transport at the cellular level | en |
dc.subject | colon tumor | en |
dc.subject | xenograft | en |
dc.subject | cell culture | en |
dc.subject | genistein | en |
dc.subject | Tumor Cells, Cultured | en |
dc.subject | Mice, Nude | en |
dc.subject | nude mouse | en |
dc.subject | binding site | en |
dc.subject | Antibodies, Monoclonal | en |
dc.subject | antibody conjugate | en |
dc.subject | Binding Sites | en |
dc.subject | genistein 17.1A immunoconjugate | en |
dc.subject | genistein-17.1A immunoconjugate | en |
dc.subject | Immunoconjugates | en |
dc.subject | Transplantation, Heterologous | en |
dc.title | Targeting colon cancer cells with genistein-17.1A immunoconjugate. | en |
dc.type | info:eu-repo/semantics/article | |
dc.description.volume | 22 | |
dc.description.startingpage | 955 | |
dc.description.endingpage | 959 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :5</p> | en |
dc.source.abbreviation | Int.J.Oncol. | en |
dc.contributor.orcid | Constantinou, Andreas I. [0000-0003-0365-1821] | |
dc.gnosis.orcid | 0000-0003-0365-1821 | |