Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1-infected individuals on prolonged effective antiretroviral therapy
Date
1999Author
Lewin, S. R.Vesanen, M.
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Hurley, A.
Duran, M.
Zhang, L.
Ho, David D.
Markowitz, M.
Gibson, U. E.
Heid, C. A.
ISSN
0022-538XSource
Journal of virologyVolume
73Pages
6099-6103Google Scholar check
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We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (WS) or partially suppressed, based on longitudinal measurements of plasma HIV-1 RNA. The proportion of individuals with US mRNA undetectable over time was significantly higher among WS individuals however, 30% of WS subjects still had detectable US mRNA after 24 months of effective antiviral therapy.
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