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dc.contributor.authorStylianou, Panayiotaen
dc.contributor.authorSkourides, Paris A.en
dc.creatorStylianou, Panayiotaen
dc.creatorSkourides, Paris A.en
dc.date.accessioned2019-11-04T12:52:45Z
dc.date.available2019-11-04T12:52:45Z
dc.date.issued2009
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/53411
dc.description.abstractMesoderm migration is a well studied morphogenetic movement that takes place during Xenopus gastrulation. The study of mesoderm migration and other morphogenetic movements has been primarily based on in vitro assays due to the inability to image deep tissue movements in the opaque embryo. We are the first to report the use of Near Infra Red Quantum Dots (NIR QD's) to image mesoderm migration in vivo with single cell resolution and provide quantitative in vivo data regarding migration rates. In addition we use QD's to address the function of the focal adhesion kinase (FAK) in this movement. Inhibition of FAK blocks mesoderm spreading and migration both in vitro and in vivo without affecting convergent extension highlighting the molecular differences between the two movements. These results provide new insights about the role of FAK and of focal adhesions during gastrulation and provide a new tool for the study of morphogenesis in vivo. © 2009 Elsevier Ireland Ltd. All rights reserved.en
dc.sourceMechanisms of developmenten
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-70349543966&doi=10.1016%2fj.mod.2009.07.008&partnerID=40&md5=7258aba7c3f419e7910fb5bff875cbc3
dc.subjectarticleen
dc.subjectcontrolled studyen
dc.subjectpriority journalen
dc.subjectquantitative analysisen
dc.subjectnonhumanen
dc.subjectAnimalsen
dc.subjectanimal tissueen
dc.subjectin vitro studyen
dc.subjectcell migrationen
dc.subjectenzyme activityen
dc.subjectenzyme inhibitionen
dc.subjectNanoparticlesen
dc.subjectnanocrystalen
dc.subjectin vivo studyen
dc.subjectquantum doten
dc.subjectQuantum Dotsen
dc.subjectembryoen
dc.subjectXenopusen
dc.subjectFocal Adhesion Protein-Tyrosine Kinasesen
dc.subjectFocal adhesionsen
dc.subjectXenopus laevisen
dc.subjectfocal adhesionen
dc.subjectfocal adhesion kinaseen
dc.subjectmesodermen
dc.subjectmorphogenesisen
dc.subjectGastrulationen
dc.subjectfluorescence analysisen
dc.subjectFAKen
dc.subjectcell spreadingen
dc.titleImaging morphogenesis, in Xenopus with Quantum Dot nanocrystalsen
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1016/j.mod.2009.07.008
dc.description.volume126
dc.description.startingpage828
dc.description.endingpage841
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeArticleen
dc.description.notes<p>Cited By :18</p>en
dc.source.abbreviationMech.Dev.en
dc.contributor.orcidSkourides, Paris A. [0000-0003-3502-5729]
dc.gnosis.orcid0000-0003-3502-5729


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