dc.contributor.author | Marion, R. M. | en |
dc.contributor.author | Strati, Katerina | en |
dc.contributor.author | Li, H. | en |
dc.contributor.author | Tejera, A. | en |
dc.contributor.author | Schoeftner, S. | en |
dc.contributor.author | Ortega, S. | en |
dc.contributor.author | Serrano, M. | en |
dc.contributor.author | Blasco, M. A. | en |
dc.creator | Marion, R. M. | en |
dc.creator | Strati, Katerina | en |
dc.creator | Li, H. | en |
dc.creator | Tejera, A. | en |
dc.creator | Schoeftner, S. | en |
dc.creator | Ortega, S. | en |
dc.creator | Serrano, M. | en |
dc.creator | Blasco, M. A. | en |
dc.date.accessioned | 2019-11-04T12:52:19Z | |
dc.date.available | 2019-11-04T12:52:19Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 1934-5909 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53239 | |
dc.description.abstract | Telomere shortening is associated with organismal aging. iPS cells have been recently derived from old patients | en |
dc.description.abstract | however, it is not known whether telomere chromatin acquires the same characteristics as in ES cells. We show here that telomeres are elongated in iPS cells compared to the parental differentiated cells both when using four (Oct3/4, Sox2, Klf4, cMyc) or three (Oct3/4, Sox2, Klf4) reprogramming factors and both from young and aged individuals. We demonstrate genetically that, during reprogramming, telomere elongation is usually mediated by telomerase and that iPS telomeres acquire the epigenetic marks of ES cells, including a low density of trimethylated histones H3K9 and H4K20 and increased abundance of telomere transcripts. Finally, reprogramming efficiency of cells derived from increasing generations of telomerase-deficient mice shows a dramatic decrease in iPS cell efficiency, a defect that is restored by telomerase reintroduction. Together, these results highlight the importance of telomere biology for iPS cell generation and functionality. © 2009 Elsevier Inc. All rights reserved. | en |
dc.source | Cell Stem Cell | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-58949094552&doi=10.1016%2fj.stem.2008.12.010&partnerID=40&md5=7197d1fb9ce38b54dbb37b030e1ad686 | |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | controlled study | en |
dc.subject | priority journal | en |
dc.subject | Aging | en |
dc.subject | age distribution | en |
dc.subject | unclassified drug | en |
dc.subject | nonhuman | en |
dc.subject | human cell | en |
dc.subject | Animals | en |
dc.subject | Mice | en |
dc.subject | animal cell | en |
dc.subject | animal experiment | en |
dc.subject | mouse | en |
dc.subject | Pluripotent Stem Cells | en |
dc.subject | cell differentiation | en |
dc.subject | enzyme activity | en |
dc.subject | Proto-Oncogene Proteins c-myc | en |
dc.subject | epigenetics | en |
dc.subject | Fibroblasts | en |
dc.subject | Mus | en |
dc.subject | embryo | en |
dc.subject | Enzyme Activation | en |
dc.subject | Mice, Knockout | en |
dc.subject | Cells, Cultured | en |
dc.subject | embryonic stem cell | en |
dc.subject | cell line | en |
dc.subject | induced pluripotent stem cell | en |
dc.subject | pluripotent stem cell | en |
dc.subject | kruppel like factor 4 | en |
dc.subject | nuclear reprogramming | en |
dc.subject | octamer transcription factor 4 | en |
dc.subject | transcription factor Sox2 | en |
dc.subject | histone | en |
dc.subject | Embryonic Stem Cells | en |
dc.subject | Ips | en |
dc.subject | Mice, Inbred C57BL | en |
dc.subject | telomere | en |
dc.subject | chromosome size | en |
dc.subject | histone h3k9 | en |
dc.subject | histone H4K20 | en |
dc.subject | Myc protein | en |
dc.subject | STEMCELL | en |
dc.subject | telomerase | en |
dc.subject | Transplantation Chimera | en |
dc.title | Telomeres Acquire Embryonic Stem Cell Characteristics in Induced Pluripotent Stem Cells | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1016/j.stem.2008.12.010 | |
dc.description.volume | 4 | |
dc.description.startingpage | 141 | |
dc.description.endingpage | 154 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :271</p> | en |
dc.source.abbreviation | Cell Stem Cell | en |
dc.contributor.orcid | Strati, Katerina [0000-0002-2332-787X] | |
dc.gnosis.orcid | 0000-0002-2332-787X | |