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dc.contributor.authorConstantinou, Andreas I.en
dc.contributor.authorKrygier, A. E.en
dc.contributor.authorMehta, R. R.en
dc.creatorConstantinou, Andreas I.en
dc.creatorKrygier, A. E.en
dc.creatorMehta, R. R.en
dc.date.accessioned2019-11-04T12:50:22Z
dc.date.available2019-11-04T12:50:22Z
dc.date.issued1998
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/52995
dc.description.abstractResults of recent studies in animal models of mammary carcinogenesis showed that the soybean isoflavone genistein is a chemopreventive agent. The objective of the present study was to determine whether soybean isoflavones can be used for the prevention of human breast carcinogenesis. Human adenocarcinoma cells that are either estrogen-receptor positive (such as MCF- 7) or estrogen-receptor negative (such as MDA-MB-468) were used as our model system. Treatment of these cells with genistein concentrations of 15, 30, and 45 μmol/L resulted in cell growth inhibition, which was accompanied by the expression of maturation markers. Maturation was monitored by the induction of intracytoplasmic casein and lipids and the membrane protein intercellular adhesion molecule-1. These maturation markers were optimally expressed after 9 d of treatment with 30 mmol genistein/L. Both estrogen receptor-positive and -negative cells became differentiated in response to genistein treatments, suggesting that the antiestrogenic function of genistein is unrelated to the mechanism of cell differentiation. Daidzein, the other major isoflavone component of soybeans, did not induce differentiation in either MCF-7 or MDA-MB-468 cells. To explore the potential applications of this result, we used the nude mouse xenograft model of carcinogenesis. Treatment of either cell line with genistein before implantation into nude mice diminished the cells' tumorigenic potential. These data suggest that initiation of the differentiation program provides a protective effect against tumor growth in mouse xenografts.en
dc.sourceAmerican Journal of Clinical Nutritionen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0031740852&partnerID=40&md5=782cb2b2a699a94c794f9a6bbfd15cbd
dc.subjecthumanen
dc.subjectAdenocarcinomaen
dc.subjectHumansen
dc.subjectbreast canceren
dc.subjectBreast Neoplasmsen
dc.subjectcontrolled studyen
dc.subjectconference paperen
dc.subjectCell Divisionen
dc.subjectAnticarcinogenic Agentsen
dc.subjectChemopreventionen
dc.subjectnonhumanen
dc.subjecthuman cellen
dc.subjectAnimalsen
dc.subjectMiceen
dc.subjectanimal experimenten
dc.subjectanimal modelen
dc.subjectmouseen
dc.subjectlipiden
dc.subjectbreast carcinogenesisen
dc.subjectcell differentiationen
dc.subjectcancer cell cultureen
dc.subjectmembrane proteinen
dc.subjectEstrogen receptoren
dc.subjectcancer inhibitionen
dc.subjectconcentration responseen
dc.subjecttumor xenograften
dc.subjectAnimaliaen
dc.subjectintercellular adhesion molecule 1en
dc.subjectPhenotypeen
dc.subjectisoflavoneen
dc.subjectsoybeanen
dc.subjectTumor Cells, Cultureden
dc.subjectestradiolen
dc.subjectIsoflavonesen
dc.subjectcell maturationen
dc.subjectGenisteinen
dc.subjectBreast cancer cellsen
dc.subjectcaseinen
dc.subjectCell Transformation, Neoplasticen
dc.subjectDaidzeinen
dc.subjectDifferentiation programen
dc.subjectGlycine maxen
dc.subjectMammary Neoplasms, Experimentalen
dc.subjectMCF-7 cellsen
dc.subjectMDA-MB-468 cellsen
dc.subjectMice, Inbred BALB Cen
dc.subjectMice, Nudeen
dc.subjectMink cell focus-forming virusen
dc.subjectMus musculusen
dc.subjectnude mouseen
dc.subjectXenograften
dc.titleGenistein induces maturation of cultured human breast cancer cells and prevents tumor growth in nude miceen
dc.typeinfo:eu-repo/semantics/article
dc.description.volume68
dc.description.startingpage1426S
dc.description.endingpage1430S
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeArticleen
dc.description.notes<p>Manufacturers: indofine, United Statesen
dc.description.notesinnovative research, United Statesen
dc.description.notesCited By :98</p>en
dc.source.abbreviationAm.J.Clin.Nutr.en
dc.contributor.orcidConstantinou, Andreas I. [0000-0003-0365-1821]
dc.gnosis.orcid0000-0003-0365-1821


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